Isolation of Mononuclear Cells from Tonsillar Tissue
dc.contributor.author | Johnston, Andrew | |
dc.contributor.author | Sigurdardottir, Sigrun L. | |
dc.contributor.author | Ryon, Judith J. | |
dc.date.accessioned | 2020-01-13T15:13:10Z | |
dc.date.available | 2020-01-13T15:13:10Z | |
dc.date.issued | 2009-08 | |
dc.identifier.citation | Johnston, Andrew; Sigurdardottir, Sigrun L.; Ryon, Judith J. (2009). "Isolation of Mononuclear Cells from Tonsillar Tissue." Current Protocols in Immunology 86(1): 7.8.1-7.8.4. | |
dc.identifier.issn | 1934-3671 | |
dc.identifier.issn | 1934-368X | |
dc.identifier.uri | https://hdl.handle.net/2027.42/152917 | |
dc.description.abstract | Located on the inside of the throat, the paired palatine tonsils form part of the first major barrier protecting the digestive and respiratory tracts from potentially invading microorganisms. The tonsils have a surface of stratified squamous epithelium that extends into deep and branched crypts lined by reticulated epithelium, which in parts may only be one cell thick. Organized in the sub‐epithelial space are B cell rich lymphoid follicles. T cells are mostly located in the extra‐follicular spaces with a very high CD4:CD8 T cell ratio. In addition to the T and B cell subsets, six phenotypes of dendritic cells (DC) have been identified in the tonsils: Langerhans cells in the squamous epithelium, germinal center DC, and follicular DC in the germinal center, and another three DC phenotypes that are located in the extra‐follicular area (interdigitating DC, plasmacytoid DC, and lympho‐epithelial symbiosis‐DC). Here, we describe the isolation of tonsil mononuclear cells from fresh human tonsil. Curr. Protoc. Immunol. 86:7.8.1‐7.8.4. © 2009 by John Wiley & Sons, Inc. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | tonsils | |
dc.subject.other | B cells | |
dc.subject.other | T cells | |
dc.subject.other | lymphocytes | |
dc.title | Isolation of Mononuclear Cells from Tonsillar Tissue | |
dc.type | Article | |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Public Health | |
dc.subject.hlbsecondlevel | Biological Chemistry | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.subject.hlbtoplevel | Science | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/152917/1/cpim0708.pdf | |
dc.identifier.doi | 10.1002/0471142735.im0708s86 | |
dc.identifier.source | Current Protocols in Immunology | |
dc.identifier.citedreference | Hart, D. and McKenzie, J. 1988. Isolation and characterization of human tonsil dendritic cells. J. Exp. Med. 168: 157 ‐ 170. | |
dc.identifier.citedreference | Watanabe et al., 1974.See above. | |
dc.identifier.citedreference | Watanabe, T., Yoshizaki, K., Yagura, T., and Yamamura, Y. 1974. In vitro antibody formation by human tonsil lymphocytes. J. Immunol. 113: 608 ‐ 616. | |
dc.identifier.citedreference | Strowig, T., Brilot, F., Array, F., Bougras, G., Thomas, D., Muller, W.A., and Munz, C. 2008. Tonsillar NK cells restrict B cell transformation by the Epstein‐Barr virus via IFN‐gamma. PloS Pathog. 4: e27. | |
dc.identifier.citedreference | Summers, K.L., Hock, B.D., McKenzie, J.L. and Hart, D.N. 2001. Phenotypic characterization of five dendritic cell subsets in human tonsils. Am. J. Pathol. 159: 285 ‐ 295. | |
dc.identifier.citedreference | Sloyer, J., Veltri, R., and Sprinkle, P. 1973. In vitro IgM antibody synthesis by human tonsil‐derived lymphocytes. J. Immunol. 111: 183 ‐ 188. | |
dc.identifier.citedreference | Nakagawa, T., Nakagawa, N., Ambrus, J.L., and Fauci, A.S. 1988. Differential effects of Interleukin 2 vs. B cell growth factor on human B cells. J. Immunol. 140: 465 ‐ 469. | |
dc.identifier.citedreference | Muraguchi, A., Butler, J., Kehrl, J., and Fauci, A.S. 1983. Differential sensitivity of human B cell subsets to activation signals delivered by anti‐µ antibody and proliferative signals delivered by a monoclonal B cell growth factor. J. Exp. Med. 157: 530 ‐ 546. | |
dc.identifier.citedreference | Hoffmann, M., Schmidt, D., and Oettgen, H. 1973. Production of antibody to sheep red blood cells by human tonsil cells in vitro. Nature 243: 408 ‐ 410. | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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