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Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA
dc.contributor.authorPires, Richard M.
dc.contributor.authorColeman, Robert S.
dc.date.accessioned2020-01-13T15:13:14Z
dc.date.available2020-01-13T15:13:14Z
dc.date.issued2001-09
dc.identifier.citationPires, Richard M.; Coleman, Robert S. (2001). "Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA." Current Protocols in Nucleic Acid Chemistry 6(1): 5.5.1-5.5.6.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152921
dc.description.abstractThis unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis‐electrophilic tethers to form site‐specific cross‐links with a complementary DNA target. These modified oligodeoxynucleotides provide a flexible tool for studies directed at biophysical investigation of oligonucleotide structure, as well as investigation of DNA hybridization, stability, and sequence‐dependent base nucleophilicity.
dc.publisherWiley Periodicals, Inc.
dc.titleUse of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152921/1/cpnc0505.pdf
dc.identifier.doi10.1002/0471142700.nc0505s06
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceColeman, R.S. and Kesicki, E.A. 1994. Synthesis and postsynthetic modification of oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine (d S4 U). J. Am. Chem. Soc. 116: 11636 ‐ 11642.
dc.identifier.citedreferenceColeman, R.S. and Kesicki, E.A. 1995. Template‐directed cross‐linking of oligonucleotides: Site specific covalent modification of dG‐N7 within duplex DNA. J. Org. Chem. 60: 6252 ‐ 6253.
dc.identifier.citedreferenceColeman, R.S. and Pires, R.M. 1997. Covalent cross‐linking of duplex DNA using 4‐thio‐2′‐deoxyuridine as a readily modifiable platform for introduction of reactive functionalities into oligonucleotides. Nucl. Acids Res. 25: 4771 ‐ 4777.
dc.identifier.citedreferenceColeman, R.S. and Siedlecki, J.M. 1991. Synthesis and stability of S‐cyanoethyl protected thiouridine and 2′‐deoxy‐4‐thiouridine. Tetrahedron Lett. 32: 3033 ‐ 3034.
dc.identifier.citedreferenceSkinner, W.A., Cory, M., Shellenberger, T.E., and Degraw, J.I. 1967. Effect of organic compounds on reproductive processes. V: Alkylating agents derived from aryl‐, aralkyl‐, and cyclohexylmethylenediamines. J. Med. Chem. 10: 120 ‐ 121.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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