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Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
dc.contributor.authorBlazer, Levi L.
dc.contributor.authorRoman, David L.
dc.contributor.authorMuxlow, Molly R.
dc.contributor.authorNeubig, Richard R.
dc.date.accessioned2020-01-13T15:13:48Z
dc.date.available2020-01-13T15:13:48Z
dc.date.issued2010-01
dc.identifier.citationBlazer, Levi L.; Roman, David L.; Muxlow, Molly R.; Neubig, Richard R. (2010). "Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions." Current Protocols in Cytometry 51(1): 13.11.1-13.11.15.
dc.identifier.issn1934-9297
dc.identifier.issn1934-9300
dc.identifier.urihttps://hdl.handle.net/2027.42/152948
dc.description.abstractA method is described for the quantitative analysis of protein‐protein interactions using the flow cytometry protein interaction assay (FCPIA). This method is based upon immobilizing protein on a polystyrene bead, incubating these beads with a fluorescently labeled binding partner, and assessing the sample for bead‐associated fluorescence in a flow cytometer. This method can be used to calculate protein‐protein interaction affinities or to perform competition experiments with unlabeled binding partners or small molecules. Examples described in this protocol highlight the use of this assay in the quantification of the affinity of binding partners of the regulator of G‐protein signaling protein, RGS19, in either a saturation or a competition format. An adaptation of this method that is compatible for high‐throughput screening is also provided. Curr. Protoc. Cytom. 51:13.11.1‐13.11.15. © 2010 by John Wiley & Sons, Inc.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherprotein‐protein interaction
dc.subject.otherhigh‐throughput screening
dc.subject.othermultiplexing
dc.subject.otherFCPIA
dc.subject.otherRGS
dc.subject.otherG protein
dc.titleUse of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152948/1/cpcy1311.pdf
dc.identifier.doi10.1002/0471142956.cy1311s51
dc.identifier.sourceCurrent Protocols in Cytometry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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