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Amplification Using CHO Cell Expression Vectors
dc.contributor.authorKingston, Robert E.
dc.contributor.authorKaufman, Randal J.
dc.contributor.authorBebbington, C.R.
dc.contributor.authorRolfe, M.R.
dc.date.accessioned2020-01-13T15:14:09Z
dc.date.available2020-01-13T15:14:09Z
dc.date.issued2002-10
dc.identifier.citationKingston, Robert E.; Kaufman, Randal J.; Bebbington, C.R.; Rolfe, M.R. (2002). "Amplification Using CHO Cell Expression Vectors." Current Protocols in Molecular Biology 60(1): 16.23.1-16.23.13.
dc.identifier.issn1934-3639
dc.identifier.issn1934-3647
dc.identifier.urihttps://hdl.handle.net/2027.42/152963
dc.description.abstractThe ability to select for integration of plasmid DNA into the host chromosome allows the generation of stably transfected cell lines. With transfection of a selectable marker linked to a nonselectable target gene (or by cotransfection of the two unlinked genes), high‐level expression of the desired gene is obtained by selecting for amplification of the selectable marker. This unit presents two systems for gene amplification and expression. The first describes the dihydrofolate reductase (DHFR) selection system while the second is based on selection of the glutamine synthetase (GS) gene. The DHFR system is probably more widely used, and results in very high levels of amplification and expression; however, the DHFR amplification process is lengthy and may require several months to isolate and characterize a stable, amplified line. In contrast, the GS system typically requires only a single round of selection for amplification to achieve maximal expression levels.
dc.publisherAcademic Press
dc.publisherWiley Periodicals, Inc.
dc.titleAmplification Using CHO Cell Expression Vectors
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelGenetics
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152963/1/cpmb1623.pdf
dc.identifier.doi10.1002/0471142727.mb1623s60
dc.identifier.sourceCurrent Protocols in Molecular Biology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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