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Derivatization of Glass and Polypropylene Surfaces
dc.contributor.authorBeier, Markus
dc.contributor.authorHoheisel, Jörg D.
dc.date.accessioned2020-01-13T15:15:10Z
dc.date.available2020-01-13T15:15:10Z
dc.date.issued2004-09
dc.identifier.citationBeier, Markus; Hoheisel, Jörg D. (2004). "Derivatization of Glass and Polypropylene Surfaces." Current Protocols in Nucleic Acid Chemistry 18(1): 12.4.1-12.4.8.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153005
dc.description.abstractThis unit describes the derivatization of solid support media, particularly glass and polypropylene, with a linker system by an iterative process that forms a dendrimeric structure, thereby increasing stepwise the loading capacity of the surface. The procedure permits the production of various types of linkers whose characteristics can be tailored to the requirements of the eventual applications. The specific modifications described here are used either for attaching prefabricated DNA oligonucleotides, PCR products, and peptide nucleic acid (PNA) oligomers, or for in situ synthesis of DNA microarrays.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherlinker
dc.subject.otherimmobilization of biomolecules
dc.subject.othermicroarray
dc.subject.othersilanization
dc.subject.otheretching
dc.subject.othersolid support
dc.titleDerivatization of Glass and Polypropylene Surfaces
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153005/1/cpnc1204.pdf
dc.identifier.doi10.1002/0471142700.nc1204s18
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceDiehl, F., Grahlmann, S., Beier, M., and Hoheisel, J.D. 2001. Manufacturing DNA‐microarrays of high spot homogeneity and reduced background signal. Nucl. Acids Res. 29: e38.
dc.identifier.citedreferenceBeier, M. and Hoheisel, J.D. 1999. Versatile derivatisation of solid support media for covalent bonding on DNA‐microchips. Nucl. Acids Res. 27: 1970 ‐ 1977.
dc.identifier.citedreferencePease, A.C., Solas, D., Sullivan, E.J., Cronin, M.T., Holmes, C.P., and Fodor, S.P.A. 1994. Light‐generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. U.S.A. 91: 5022 ‐ 5026.
dc.identifier.citedreferenceBeier, M. and Hoheisel, J.D. 2000. Production of quantitative photolithographic synthesis of individually quality checked DNA microarrays. Nucl. Acids Res. 28: e11.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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