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CE‐MS with electrokinetic supercharging and application to determination of neurotransmitters
dc.contributor.authorWells, Shane S.
dc.contributor.authorDawod, Mohamed
dc.contributor.authorKennedy, Robert T.
dc.date.accessioned2020-01-13T15:17:24Z
dc.date.availableWITHHELD_11_MONTHS
dc.date.available2020-01-13T15:17:24Z
dc.date.issued2019-11
dc.identifier.citationWells, Shane S.; Dawod, Mohamed; Kennedy, Robert T. (2019). "CE‐MS with electrokinetic supercharging and application to determination of neurotransmitters." ELECTROPHORESIS 40(22): 2946-2953.
dc.identifier.issn0173-0835
dc.identifier.issn1522-2683
dc.identifier.urihttps://hdl.handle.net/2027.42/153096
dc.description.abstractElectrokinetic supercharging (EKS) is known as one of the most effective online electrophoretic preconcentration techniques, though pairing with it with mass spectrometry has presented challenges. Here, EKS is successfully paired with ESI‐MS/MS to provide a sensitive and robust method for analysis of biogenic amines in biological samples. Injection parameters including electric field strength and the buffer compositions used for the separation and focusing were investigated to achieve suitable resolution, high sensitivity, and compatibility with ESI‐MS. Using EKS, the sensitivity of the method was improved 5000‐fold compared to a conventional hydrodynamic injection with CZE. The separation allowed for baseline resolution of several neurotransmitters within 16 min with LODs down to 10 pM. This method was applied to targeted analysis of seven biogenic amines from rat brain stem and whole Drosophila tissue. This is the first method to use EKS with CE‐ESI‐MS/MS to analyze biological samples.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherNeurotransmitters
dc.subject.otherBiological samples
dc.subject.otherCE
dc.subject.otherCE‐MS
dc.subject.otherElectrokinetic supercharging
dc.titleCE‐MS with electrokinetic supercharging and application to determination of neurotransmitters
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153096/1/elps7049.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153096/2/elps7049_am.pdf
dc.identifier.doi10.1002/elps.201900203
dc.identifier.sourceELECTROPHORESIS
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