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Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
dc.contributor.authorKutyavin, Igor
dc.contributor.authorLokhov, Sergey
dc.contributor.authorLukhtanov, Eugene
dc.contributor.authorReed, Michael W.
dc.date.accessioned2020-01-13T15:17:29Z
dc.date.available2020-01-13T15:17:29Z
dc.date.issued2003-06
dc.identifier.citationKutyavin, Igor; Lokhov, Sergey; Lukhtanov, Eugene; Reed, Michael W. (2003). "Chemistry of Minor Groove Binder–Oligonucleotide Conjugates." Current Protocols in Nucleic Acid Chemistry 13(1): 8.4.1-8.4.21.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153100
dc.description.abstractVarious types of minor groove binders have been attached to synthetic oligodeoxynucleotides, and the interactions of these conjugates (MB‐ODNs) with DNA are reviewed here. MB‐ODNs have enhanced DNA affinity and have improved the hybridization properties of sequence‐specific DNA probes. Short MB‐ODNs hybridize with ssDNA to give more stable DNA duplexes than unmodified ODNs with similar lengths. Mismatch discrimination of short MB‐ODNs is enhanced in comparison to longer unmodified ODNs. The stronger binding of MB‐ODNs allows for more stringent hybridization conditions to be used in DNA probe‐based assays. MB‐ODNs are especially useful in quantitative “real‐time” PCR assays since they bind efficiently during the high‐temperature primer extension cycle. The synthesis and biophysical chemistry of MB‐ODN conjugates are reviewed here. Four published structural classes of MB‐ODNs and their various dsDNA binding modes are discussed, and the well‐characterized DPI3‐type MB‐ODNs and their interactions with ssDNA target strands are described in detail.
dc.publisherCRC Press
dc.publisherWiley Periodicals, Inc.
dc.titleChemistry of Minor Groove Binder–Oligonucleotide Conjugates
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153100/1/cpnc0804.pdf
dc.identifier.doi10.1002/0471142700.nc0804s13
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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