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Analysis and Purification of Synthetic Nucleic Acids Using HPLC
dc.contributor.authorAndrus, Alex
dc.contributor.authorKuimelis, Robert G.
dc.date.accessioned2020-01-13T15:17:37Z
dc.date.available2020-01-13T15:17:37Z
dc.date.issued2000-07
dc.identifier.citationAndrus, Alex; Kuimelis, Robert G. (2000). "Analysis and Purification of Synthetic Nucleic Acids Using HPLC." Current Protocols in Nucleic Acid Chemistry 1(1): 10.5.1-10.5.13.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153106
dc.description.abstractHPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed‐phase HPLC allows a high‐capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion‐exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed‐phase and anion‐exchange HPLC, it can be used for oligonucleotides of up to ˜50 nt in length.
dc.publisherWiley Periodicals, Inc.
dc.publisherStockton Press
dc.titleAnalysis and Purification of Synthetic Nucleic Acids Using HPLC
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153106/1/cpnc1005.pdf
dc.identifier.doi10.1002/0471142700.nc1005s01
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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