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Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands
dc.contributor.authorAsseline, Ulysse
dc.contributor.authorThuong, Nguyen T.
dc.date.accessioned2020-01-13T15:17:41Z
dc.date.available2020-01-13T15:17:41Z
dc.date.issued2001-12
dc.identifier.citationAsseline, Ulysse; Thuong, Nguyen T. (2001). "Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands." Current Protocols in Nucleic Acid Chemistry 7(1): 4.10.1-4.10.23.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153109
dc.description.abstractThis unit reports the synthesis of oligodeoxyribonucleotides covalently linked via their 5′ termini to various ligands such as intercalating agents, reactive groups, or labels. Methods for incorporation of halogenoalkyl, isothiocyanate, and 2‐pyridyldisulfide linkers onto ligands, and addition of amino, carboxyl, thiophosphate, phosphate, and masked thiol groups at the 5 terminus of an oligodeoxyribonucleotide are described elsewhere in the series. This unit reports procedures for coupling the ligands and oligonucleotides, as well as details for purification and characterization of the final products.
dc.publisherIRL Press
dc.publisherWiley Periodicals, Inc.
dc.titleConjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153109/1/cpnc0410.pdf
dc.identifier.doi10.1002/0471142700.nc0410s07
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceAsseline, U., Bonfils, E., Dupret, D., and Thuong, N.T. 1996. Synthesis and binding properties of oligonucleotides covalently linked to an acridine derivative: New study of the influence of the dye attachment site. Bioconjugate Chem. 7: 369 ‐ 379.
dc.identifier.citedreferenceAubert, Y., Bourgerie, S., Meunier, L., Mayer, R., Roche, A.‐C., Monsigny, M., Thuong, N.T., and Asseline, U. 2000. Optimized synthesis of phosphorothioate oligodeoxyribonucleotides substituted with a 5′‐protected thiol function and a 3′‐amino group. Nucl. Acids Res. 28: 818 ‐ 825.
dc.identifier.citedreferenceAubert, Y., Perrouault, L., Hélene, C., Giovannangeli, C., and Asseline, U. 2001. Synthesis and properties of triple helix‐forming oligodeoxyribonucleotides containing 7‐chloro‐7‐deaza‐2′‐deoxyguanosine. Bioorg. Med. Chem. 9: 1617 ‐ 1624.
dc.identifier.citedreferenceIvanovskaya, M.G., Gottihk, M.B., and Shabarova, Z.A. 1987. Modification of Oligo(poly)nucleotide Phosphomonoester groups in aqueous solutions. Nucleosides‐Nucleotides 6: 913 ‐ 934.
dc.identifier.citedreferenceRaymond, F., Asseline, U., Roig, V., and Thuong, N.T. 1996. Synthesis and characterization of O 6 modified deoxyguanosine‐containing oligodeoxyribonucleotides for triple helix formation. Tetrahedon 52: 2047 ‐ 2064.
dc.identifier.citedreferenceSmith, L.M., Fung, S., Hunkapiller, M.W., Hunkapiller, T.J., and Hood, L.E. 1985. The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: Synthesis of fluorescent DNA primers for use in DNA analysis. Nucl. Acids. Res. 13: 2399 ‐ 2412.
dc.identifier.citedreferenceThuong, N.T. and Asseline, U. 1991. Oligodeoxyribonucleotides attached to intercalators, photoreactive and cleavage agents. In Oligodeoxyribonucleotides and Analogues: A Practical Approach ( F. Eckstein, ed.) pp. 283 ‐ 308. IRL Press, Oxford.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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