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Selection of Transfected Mammalian Cells
dc.contributor.authorMortensen, Richard M.
dc.contributor.authorKingston, Robert E.
dc.date.accessioned2020-01-13T15:18:46Z
dc.date.available2020-01-13T15:18:46Z
dc.date.issued2009-04
dc.identifier.citationMortensen, Richard M.; Kingston, Robert E. (2009). "Selection of Transfected Mammalian Cells." Current Protocols in Molecular Biology 86(1): 9.5.1-9.5.13.
dc.identifier.issn1934-3639
dc.identifier.issn1934-3647
dc.identifier.urihttps://hdl.handle.net/2027.42/153151
dc.description.abstractTo determine the function of a gene in vitro, expression in heterologous cells is often employed. This can be done by transient expression, but often requires a more permanent expression of the gene and the creation of a cell line. This process can involve decisions as to the nature of construct used for expression, and invariably uses some strategy to select the transfected cells. Typically, these strategies use one of a number of genes that confer resistance to an added drug that will kill untransfected cells but not the transfected cells (positive selection). Alternatively, sometimes the strategy uses a gene that will confer sensitivity to a compound and kills the transfected cells (negative selection). This chapter discusses some of the strategies and genes used in creating cell line for in vitro study of gene function. Curr. Protoc. Mol. Biol. 86:9.5.1‐9.5.13. © 2009 by John Wiley & Sons, Inc.
dc.publisherIRL Press
dc.publisherWiley Periodicals, Inc.
dc.subject.otherFlp‐FRT
dc.subject.otherstable integration
dc.subject.otherselection
dc.subject.othermammalian cell
dc.subject.otherstable transfection
dc.subject.otherselection marker
dc.subject.otherCre‐lox
dc.subject.otherexpression
dc.titleSelection of Transfected Mammalian Cells
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelGenetics
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153151/1/cpmb0905.pdf
dc.identifier.doi10.1002/0471142727.mb0905s86
dc.identifier.sourceCurrent Protocols in Molecular Biology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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