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Rapid Magnesium Chelation as a Method to Study Real‐Time Tertiary Unfolding of RNA
dc.contributor.authorMaglott, Emily J.
dc.contributor.authorGlick, Gary D.
dc.date.accessioned2020-01-13T15:19:12Z
dc.date.available2020-01-13T15:19:12Z
dc.date.issued2001-09
dc.identifier.citationMaglott, Emily J.; Glick, Gary D. (2001). "Rapid Magnesium Chelation as a Method to Study Real‐Time Tertiary Unfolding of RNA." Current Protocols in Nucleic Acid Chemistry 6(1): 11.7.1-11.7.11.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153169
dc.description.abstractThis unit describes a method to measure the unfolding of RNA tertiary structure on a millisecond time scale. A stopped‐flow spectrophotometer is used to measure the rate of unfolding induced by the addition of EDTA to an RNA whose tertiary structure has been stabilized in the presence of magnesium ions. Using this methodology, rate constants for unfolding of tertiary or secondary structure can be obtained over a range of temperatures, and these values can be used to construct Arrhenius and Eyring plots, from which activation energy, Arrhenius pre‐exponential factor, and enthalpy and entropy of activation can be obtained. These data provide information about the energy of the transition state and the energy barriers between secondary and tertiary structure, which is necessary for predicting RNA tertiary structure from secondary structure.
dc.publisherWiley Periodicals, Inc.
dc.publisherMIT Press
dc.titleRapid Magnesium Chelation as a Method to Study Real‐Time Tertiary Unfolding of RNA
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153169/1/cpnc1107.pdf
dc.identifier.doi10.1002/0471142700.nc1107s06
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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