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2′‐Hydroxyl‐Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions
dc.contributor.authorMiller, Tod J.
dc.contributor.authorSchwartz, Miriam E.
dc.contributor.authorGough, Geoffrey R.
dc.date.accessioned2020-01-13T15:19:28Z
dc.date.available2020-01-13T15:19:28Z
dc.date.issued2000-12
dc.identifier.citationMiller, Tod J.; Schwartz, Miriam E.; Gough, Geoffrey R. (2000). "2′‐Hydroxyl‐Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions." Current Protocols in Nucleic Acid Chemistry 3(1): 2.5.1-2.5.36.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153181
dc.description.abstractProtected ribonucleotide monomers are more difficult to obtain than their 2′‐deoxy counterparts because of the need to protect the 2′‐hydroxy function. This unit describes the stepwise preparation of suitably 2′‐protected ribonucleosides using two protecting groups: 2‐nitrobenzyloxymethyl (NBOM) and tert‐butyldimethylsilyl (TBDMS). In addition, details are given for protecting the 5′‐hydroxyl and the nucleobase, yielding nucleosides that are easily converted to phosphoramidite or H‐phosphonate derivatives for automated oligoribonucleotide synthesis.
dc.publisherWiley Periodicals, Inc.
dc.title2′‐Hydroxyl‐Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153181/1/cpnc0205.pdf
dc.identifier.doi10.1002/0471142700.nc0205s03
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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