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Chemical Synthesis of RNA Sequences with 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleoside Phosphoramidites
dc.contributor.authorPitsch, Stefan
dc.contributor.authorWeiss, Patrick A.
dc.date.accessioned2020-01-13T15:21:15Z
dc.date.available2020-01-13T15:21:15Z
dc.date.issued2001-12
dc.identifier.citationPitsch, Stefan; Weiss, Patrick A. (2001). "Chemical Synthesis of RNA Sequences with 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleoside Phosphoramidites." Current Protocols in Nucleic Acid Chemistry 7(1): 3.8.1-3.8.15.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/153249
dc.description.abstractThe [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction conditions applied during assembly and the first deprotection step. It does not interfere with the coupling reaction and leads to very good coupling yields under DNA‐coupling conditions. The final cleavage occurs quantitatively without concomitant destruction of the RNA product. This unit describes the synthesis of TOM‐phosphoramidites from 2′‐O‐TOM‐5′‐O‐dimethoxytrityl‐N‐acetyl ribonucleosides, oligoribonucleotide assembly on an automated DNA synthesizer, and subsequent deprotection. Preparation of the TOM‐protected ribonucleosides is presented elsewhere in the series.
dc.publisherOxford University Press
dc.publisherWiley Periodicals, Inc.
dc.titleChemical Synthesis of RNA Sequences with 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleoside Phosphoramidites
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/153249/1/cpnc0308.pdf
dc.identifier.doi10.1002/0471142700.nc0308s07
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.identifier.citedreferencePitsch, S., Weiss, P.A., Jenny, L., Stutz, A., and Wu, X. 2001. Reliable chemical synthesis of oligoribonucleotides (RNA) with 2′‐ O ‐[(triisopropylsilyl)oxy]methyl (2′‐ O ‐TOM) protected phosphoramidites. Helv. Chim. Acta In press.
dc.identifier.citedreferenceReese, C.B., Rao, M.V., Serafinowska, H.T., Thompson, E.A., and Yu, P.S. 1991. Studies in the solid phase synthesis of oligo‐ and poly‐ribonucleotides. Nucleosides Nucleotides 10: 81 ‐ 97.
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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