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Characterization of macrophages infiltrating periâ implantitis lesions
dc.contributor.authorFretwurst, Tobias
dc.contributor.authorGaraicoa‐pazmino, Carlos
dc.contributor.authorNelson, Katja
dc.contributor.authorGiannobile, William V.
dc.contributor.authorSquarize, Cristiane H.
dc.contributor.authorLarsson, Lena
dc.contributor.authorCastilho, Rogerio M.
dc.date.accessioned2020-03-17T18:35:46Z
dc.date.availableWITHHELD_13_MONTHS
dc.date.available2020-03-17T18:35:46Z
dc.date.issued2020-03
dc.identifier.citationFretwurst, Tobias; Garaicoa‐pazmino, Carlos ; Nelson, Katja; Giannobile, William V.; Squarize, Cristiane H.; Larsson, Lena; Castilho, Rogerio M. (2020). "Characterization of macrophages infiltrating periâ implantitis lesions." Clinical Oral Implants Research 31(3): 274-281.
dc.identifier.issn0905-7161
dc.identifier.issn1600-0501
dc.identifier.urihttps://hdl.handle.net/2027.42/154542
dc.description.abstractObjectivesThe mechanisms involved in the initiation and progression of periâ implantitis lesions are poorly understood. It was the aim to determine the content and activation status of macrophages present in human periâ implantitis lesions and compare the current findings with the macrophage polarization associated with periodontitis lesions.Material and MethodsA total of 14 patients were studied in this investigation. Seven were soft tissue biopsies from dental implants affected by periâ implantitis that required explantation. Seven biopsies were from chronic periodontal disease. Immunofluorescence stains were performed using biomarkers to identify macrophages (CD68+) undergoing M1 polarization (iNOS+) and M2 polarization (CD206+), along with Hoechst 33,342 to identify DNA content. All samples were stained and photographed, and doubleâ positive cells for CD68 and iNOS or CD68 and CD206 were quantified.ResultsAll periâ implantitis biopsies examined revealed a mixed population of macrophages undergoing M1 polarization and M2 polarization. Further analysis demonstrated the coâ expression of iNOS and CD206, which indicates the presence of a heterogenic immune response on periâ implantitis lesions. Macrophage polarization in periâ implantitis lesions presents a distinct pattern than in periodontitis. We observed a significant increase in the population of M1 macrophages on periâ implantitis samples compared to periodontal disease samples.ConclusionOur results demonstrate that periâ implantitis has higher numbers of macrophages displaying a distinct macrophage M1 polarization signature compared to periodontitis lesions. This pattern may explain, in part, the distinct nature of periâ implantitis progression vs. periodontitis in humans.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherdental implants
dc.subject.otherhistology
dc.subject.otherinflammation
dc.subject.othermacrophages
dc.subject.othernitric oxide synthase
dc.subject.otherPeriâ implantitis
dc.subject.otherperiodontitis
dc.titleCharacterization of macrophages infiltrating periâ implantitis lesions
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelDentistry
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/154542/1/clr13568_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/154542/2/clr13568.pdf
dc.identifier.doi10.1111/clr.13568
dc.identifier.sourceClinical Oral Implants Research
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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