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Characterization of novel calcium hydroxide- mediated highly porous chitosan- calcium scaffolds for potential application in dentin tissue engineering
dc.contributor.authorSoares, Diana Gabriela
dc.contributor.authorBordini, Ester Alves Ferreira
dc.contributor.authorCassiano, Fernanda Balestrero
dc.contributor.authorBronze‐uhle, Erika Soares
dc.contributor.authorPacheco, Leandro Edgar
dc.contributor.authorZabeo, Giovana
dc.contributor.authorHebling, Josimeri
dc.contributor.authorLisboa‐filho, Paulo Noronha
dc.contributor.authorBottino, Marco Cicero
dc.contributor.authorSouza Costa, Carlos Alberto
dc.date.accessioned2020-07-02T20:32:52Z
dc.date.availableWITHHELD_14_MONTHS
dc.date.available2020-07-02T20:32:52Z
dc.date.issued2020-08
dc.identifier.citationSoares, Diana Gabriela; Bordini, Ester Alves Ferreira; Cassiano, Fernanda Balestrero; Bronze‐uhle, Erika Soares ; Pacheco, Leandro Edgar; Zabeo, Giovana; Hebling, Josimeri; Lisboa‐filho, Paulo Noronha ; Bottino, Marco Cicero; Souza Costa, Carlos Alberto (2020). "Characterization of novel calcium hydroxide- mediated highly porous chitosan- calcium scaffolds for potential application in dentin tissue engineering." Journal of Biomedical Materials Research Part B: Applied Biomaterials 108(6): 2546-2559.
dc.identifier.issn1552-4973
dc.identifier.issn1552-4981
dc.identifier.urihttps://hdl.handle.net/2027.42/155906
dc.description.abstractThe aim of this study was to develop a highly porous calcium- containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase- separation technique. Scanning electron microscopy/energy- dispersive spectroscopy demonstrated the fabrication of a highly porous calcium- linked chitosan scaffold (CH- Ca), with a well- organized and interconnected porous network. Scaffolds were cross- linked on glutaraldehyde (GA) vapor. Following a 28- day incubation in water, cross- linked CH scaffold had no changes on humid mass, and CH- Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28- days (42.2%). Fourier- transform infrared spectroscopy indicated the establishment of Schiff base on cross- linked scaffolds, along with calcium complexation for CH- Ca. Cross- linked CH- Ca scaffold featured a sustained Ca2+ release up to 21- days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH- Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP- 1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium- rich matrix deposition. In sum, our findings suggest that CH- Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
dc.publisherJohn Wiley & Sons, Inc.
dc.subject.othercalcium hydroxide
dc.subject.otherchitosan
dc.subject.otherdental pulp
dc.subject.otherdentin
dc.subject.otherporous scaffolds
dc.titleCharacterization of novel calcium hydroxide- mediated highly porous chitosan- calcium scaffolds for potential application in dentin tissue engineering
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbsecondlevelBiomedical Engineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/155906/1/jbmb34586.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/155906/2/jbmb34586_am.pdf
dc.identifier.doi10.1002/jbm.b.34586
dc.identifier.sourceJournal of Biomedical Materials Research Part B: Applied Biomaterials
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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