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Murine Norovirus: Propagation, Quantification, and Genetic Manipulation
dc.contributor.authorHwang, Seungmin
dc.contributor.authorAlhatlani, Bader
dc.contributor.authorArias, Armando
dc.contributor.authorCaddy, Sarah L.
dc.contributor.authorChristodoulou, Constantina
dc.contributor.authorBragazzi Cunha, Juliana
dc.contributor.authorEmmott, Ed
dc.contributor.authorGonzalez‐hernandez, Marta
dc.contributor.authorKolawole, Abimbola
dc.contributor.authorLu, Jia
dc.contributor.authorRippinger, Christine
dc.contributor.authorSorgeloos, Frédéric
dc.contributor.authorThorne, Lucy
dc.contributor.authorVashist, Surender
dc.contributor.authorGoodfellow, Ian
dc.contributor.authorWobus, Christiane E.
dc.date.accessioned2021-01-05T18:45:15Z
dc.date.available2021-01-05T18:45:15Z
dc.date.issued2014-05
dc.identifier.citationHwang, Seungmin; Alhatlani, Bader; Arias, Armando; Caddy, Sarah L.; Christodoulou, Constantina; Bragazzi Cunha, Juliana; Emmott, Ed; Gonzalez‐hernandez, Marta ; Kolawole, Abimbola; Lu, Jia; Rippinger, Christine; Sorgeloos, Frédéric ; Thorne, Lucy; Vashist, Surender; Goodfellow, Ian; Wobus, Christiane E. (2014). "Murine Norovirus: Propagation, Quantification, and Genetic Manipulation." Current Protocols in Microbiology 33(1): 15K.2.1-15K.2.61.
dc.identifier.issn1934-8525
dc.identifier.issn1934-8533
dc.identifier.urihttps://hdl.handle.net/2027.42/163819
dc.description.abstractMurine norovirus (MNV) is a positive- sense, plus- stranded RNA virus in the Caliciviridae family. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal- oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology. Curr. Protoc. Microbiol 33:15K.2.1- 15K.2.61. © 2014 by John Wiley & Sons, Inc.
dc.publisherWiley Periodicals, Inc.
dc.publisherLippincott Williams & Wilkins
dc.subject.othermurine norovirus
dc.subject.otherquantification
dc.subject.otherpurification
dc.subject.othertransfection
dc.subject.otherreverse genetics
dc.titleMurine Norovirus: Propagation, Quantification, and Genetic Manipulation
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMicrobiology and Immunology
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/163819/1/cpmc15k02.pdf
dc.identifier.doi10.1002/9780471729259.mc15k02s33
dc.identifier.sourceCurrent Protocols in Microbiology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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