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A novel method of high‐purity extracellular vesicle enrichment from microliter‐scale human serum for proteomic analysis
dc.contributor.authorJi, Xiaohui
dc.contributor.authorHuang, Sisi
dc.contributor.authorZhang, Jie
dc.contributor.authorBruce, Terri F.
dc.contributor.authorTan, Zhijing
dc.contributor.authorWang, Donglin
dc.contributor.authorZhu, Jianhui
dc.contributor.authorMarcus, R. Kenneth
dc.contributor.authorLubman, David M.
dc.date.accessioned2021-02-04T21:54:17Z
dc.date.available2022-03-04 16:54:15en
dc.date.available2021-02-04T21:54:17Z
dc.date.issued2021-02
dc.identifier.citationJi, Xiaohui; Huang, Sisi; Zhang, Jie; Bruce, Terri F.; Tan, Zhijing; Wang, Donglin; Zhu, Jianhui; Marcus, R. Kenneth; Lubman, David M. (2021). "A novel method of high‐purity extracellular vesicle enrichment from microliter‐scale human serum for proteomic analysis." ELECTROPHORESIS 42(3): 245-256.
dc.identifier.issn0173-0835
dc.identifier.issn1522-2683
dc.identifier.urihttps://hdl.handle.net/2027.42/166271
dc.description.abstractWe have developed a rapid, low‐cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,<100 μL) with minimal contamination by serum proteins and lipoprotein particles to meet the high purity requirement for EV proteome analysis. EVs were separated by a novel polyester capillary channel polymer (PET C‐CP) fiber phase/hydrophobic interaction chromatography (HIC) method which is rapid and can process small size samples. The collected EV fractions were subjected to a post‐column cleanup protocol using a centrifugal filter to perform buffer exchange and eliminate potential coeluting non‐EV proteins while minimizing EV sample loss. Downstream characterization demonstrated that our current strategy can separate EVs with the anticipated exosome‐like particle size distribution and high yield (∼1 × 1011 EV particles per mL of serum) in approximately 15 min. Proteome profiling of the EVs reveals that a group of genuine EV components were identified that have significantly less high‐abundance blood proteins and lipoprotein particle contamination in comparison to traditional separation methods. The use of this methodology appears to address the major challenges facing EV separation for proteomics analysis. In addition, the EV post‐column cleanup protocol proposed in the current work has the potential to be combined with other separation methods, such as ultracentrifugation (UC), to further purify the separated EV samples.
dc.publisherEndotext, MDText.com, Inc.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherSerum
dc.subject.otherCapillary‐channeled polymer (C‐CP) fibers
dc.subject.otherHydrophobic interaction chromatography (HIC)
dc.subject.otherMass spectrometry
dc.subject.otherProteomics
dc.subject.otherExtracellular vesicles
dc.titleA novel method of high‐purity extracellular vesicle enrichment from microliter‐scale human serum for proteomic analysis
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/166271/1/elps7307.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/166271/2/elps7307-sup-0001-SuppMat.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/166271/3/elps7307_am.pdf
dc.identifier.doi10.1002/elps.202000223
dc.identifier.doihttps://dx.doi.org/10.7302/194
dc.identifier.sourceELECTROPHORESIS
dc.identifier.citedreferenceAn, M., Zou, X., Wang, Q., Zhao, X., Wu, J., Xu, L. M., Shen, H. Y., Xiao, X., He, D., Ji, J., Anal. Chem. 2013, 85, 4530 – 4537.
dc.identifier.citedreferenceKim, J., Tan, Z., Lubman, D. M., Electrophoresis 2015, 36, 2017 – 2026.
dc.identifier.citedreferenceFeingold, K. R., Grunfeld, C., in: Feingold, K. R., Anawalt, B., Boyce, A., Chrousos, G., Dungan, K., Grossman, A., Hershman, J. M., Kaltsas, G., Koch, C., Kopp, P., Korbonits, M., McLachlan, R., Morley, J. E., New, M., Perreault, L., Purnell, J., Rebar, R., Singer, F., Trence, D. L., Vinik, A., Wilson, D. P. (Eds.), Introduction to Lipids and Lipoproteins, Endotext, MDText.com, Inc., South Dartmouth (MA)  2000.
dc.identifier.citedreferenceYuana, Y., Levels, J., Grootemaat, A., Sturk, A., Nieuwland, R., J. Extracell. Vesicles 2014, 3, 23262.
dc.identifier.citedreferenceSodar, B. W., Kittel, A., Paloczi, K., Vukman, K. V., Osteikoetxea, X., Szabo‐Taylor, K., Nemeth, A., Sperlagh, B., Baranyai, T., Giricz, Z., Wiener, Z., Turiak, L., Drahos, L., Pallinger, E., Vekey, K., Ferdinandy, P., Falus, A., Buzas, E. I., Sci. Rep. 2016, 6, 24316.
dc.identifier.citedreferenceWelton, J. L., Webber, J. P., Botos, L. A., Jones, M., Clayton, A., J. Extracell. Vesicles 2015, 4, 27269.
dc.identifier.citedreferencede Menezes‐Neto, A., Saez, M. J., Lozano‐Ramos, I., Segui‐Barber, J., Martin‐Jaular, L., Ullate, J. M., Fernandez‐Becerra, C., Borras, F. E., Del Portillo, H. A., J. Extracell. Vesicles 2015, 4, 27378.
dc.identifier.citedreferenceCao, F., Gao, Y., Chu, Q., Wu, Q., Zhao, L., Lan, T., Zhao, L., Electrophoresis 2019, 40, 3092 – 3098.
dc.identifier.citedreferenceRider, M. A., Hurwitz, S. N., Meckes, D. G., Jr., Sci. Rep. 2016, 6, 23978.
dc.identifier.citedreferenceYang, F., Liao, X., Tian, Y., Li, G., Biotechnol. J. 2017, 12, 1600699.
dc.identifier.citedreferenceWu, M., Ouyang, Y., Wang, Z., Zhang, R., Huang, P. H., Chen, C., Li, H., Li, P., Quinn, D., Dao, M., Suresh, S., Sadovsky, Y., Huang, T. J., Proc. Natl. Acad. Sci. USA 2017, 114, 10584 – 10589.
dc.identifier.citedreferenceLiu, C., Guo, J., Tian, F., Yang, N., Yan, F., Ding, Y., Wei, J., Hu, G., Nie, G., Sun, J., ACS Nano 2017, 11, 6968 – 6976.
dc.identifier.citedreferenceZhao, Z., Yang, Y., Zeng, Y., He, M., Lab Chip 2016, 16, 489 – 496.
dc.identifier.citedreferenceLewis, J. M., Vyas, A. D., Qiu, Y., Messer, K. S., White, R., Heller, M. J., ACS Nano 2018, 12, 3311 – 3320.
dc.identifier.citedreferenceLi, P., Kaslan, M., Lee, S. H., Yao, J., Gao, Z., Theranostics 2017, 7, 789 – 804.
dc.identifier.citedreferenceBruce, T. F., Slonecki, T. J., Wang, L., Huang, S., Powell, R. R., Marcus, R. K., Electrophoresis 2019, 40, 571 – 581.
dc.identifier.citedreferenceHuang, S., Wang, L., Bruce, T. F., Marcus, R. K., Anal. Bioanal. Chem. 2019, 411, 6591 – 6601.
dc.identifier.citedreferenceWang, L., Bruce, T. F., Huang, S., Marcus, R. K., Anal. Chim. Acta 2019, 1082, 186 – 193.
dc.identifier.citedreferenceHuang, S., Wang, L., Bruce, T. F., Marcus, R. K., Biotechnol. Prog. 2020, e2998.,.
dc.identifier.citedreferenceJackson, K. K., Powell, R. R., Bruce, T. F., Marcus, R. K., Anal. Bioanal. Chem. 2020, 412, 4713 – 4724.
dc.identifier.citedreferenceRandunu, K. M., Marcus, R. K., Anal. Bioanal. Chem. 2012, 404, 721 – 729.
dc.identifier.citedreferenceVergauwen, G., Dhondt, B., Van Deun, J., De Smedt, E., Berx, G., Timmerman, E., Gevaert, K., Miinalainen, I., Cocquyt, V., Braems, G., Van den Broecke, R., Denys, H., De Wever, O., Hendrix, A., Sci. Rep. 2017, 7, 2704.
dc.identifier.citedreferenceWisniewski, J. R., Methods Mol. Biol. 2018, 1841, 3 – 10.
dc.identifier.citedreferenceCox, J., Mann, M., Nat. Biotechnol. 2008, 26, 1367 – 1372.
dc.identifier.citedreferencePathan, M., Keerthikumar, S., Chisanga, D., Alessandro, R., Ang, C. S., Askenase, P., Batagov, A. O., Benito‐Martin, A., Camussi, G., Clayton, A., Collino, F., Di Vizio, D., Falcon‐Perez, J. M., Fonseca, P., Fonseka, P., Fontana, S., Gho, Y. S., Hendrix, A., Hoen, E. N., Iraci, N., Kastaniegaard, K., Kislinger, T., Kowal, J., Kurochkin, I. V., Leonardi, T., Liang, Y., Llorente, A., Lunavat, T. R., Maji, S., Monteleone, F., Øverbye, A., Panaretakis, T., Patel, T., Peinado, H., Pluchino, S., Principe, S., Ronquist, G., Royo, F., Sahoo, S., Spinelli, C., Stensballe, A., Théry, C., van Herwijnen, M. J. C., Wauben, M., Welton, J. L., Zhao, K., Mathivanan, S., J. Extracell. Vesicles 2017, 6, 1321455.
dc.identifier.citedreferenceThe Gene Ontology Consortium, Nucleic Acids Res. 2015, 43, D1049 – D1056.
dc.identifier.citedreferencePathan, M., Fonseka, P., Chitti, S. V., Kang, T., Sanwlani, R., Van Deun, J., Hendrix, A., Mathivanan, S., Nucleic Acids Res. 2019, 47, D516 – D519.
dc.identifier.citedreferenceThery, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., Ayre, D. C., Bach, J.‐M., Bachurski, D., Baharvand, H., Balaj, L., Baldacchino, S., Bauer, N. N., Baxter, A. A., Bebawy, M., Beckham, C., Bedina Zavec, A., Benmoussa, A., Berardi, A. C., Bergese, P., Bielska, E., Blenkiron, C., Bobis‐Wozowicz, S., Boilard, E., Boireau, W., Bongiovanni, A., Borràs, F. E., Bosch, S., Boulanger, C. M., Breakefield, X., Breglio, A. M., Brennan, M. Á., Brigstock, D. R., Brisson, A., Broekman, M. L. D., Bromberg, J. F., Bryl‐Górecka, P., Buch, S., Buck, A. H., Burger, D., Busatto, S., Buschmann, D., Bussolati, B., Buzás, E. I., Byrd, J. B., Camussi, G., Carter, D. R. F., Caruso, S., Chamley, L. W., Chang, Y. T., Chen, C., Chen, S., Cheng, L., Chin, A. R., Clayton, A., Clerici, S. P., Cocks, A., Cocucci, E., Coffey, R. J., Cordeiro‐da‐Silva, A., Couch, Y., Coumans, F. A. W., Coyle, B., Crescitelli, R., Criado, M. F., D’Souza‐Schorey, C., Das, S., Datta Chaudhuri, A., de Candia, P., De Santana, E. F., De Wever, O., Del Portillo, H. A., Demaret, T., Deville, S., Devitt, A., Dhondt, B., Di Vizio, D., Dieterich, L. C., Dolo, V., Dominguez Rubio, A. P., Dominici, M., Dourado, M. R., Driedonks, T. A. P., Duarte, F. V., Duncan, H. M., Eichenberger, R. M., Ekström, K., El Andaloussi, S., Elie‐Caille, C., Erdbrügger, U., Falcón‐Pérez, J. M., Fatima, F., Fish, J. E., Flores‐Bellver, M., Försönits, A., Frelet‐Barrand, A., Fricke, F., Fuhrmann, G., Gabrielsson, S., Gámez‐Valero, A., Gardiner, C., Gärtner, K., Gaudin, R., Gho, Y. S., Giebel, B., Gilbert, C., Gimona, M., Giusti, I., Goberdhan, D. C. I., Görgens, A., Gorski, S. M., Greening, D. W., Gross, J. C., Gualerzi, A., Gupta, G. N., Gustafson, D., Handberg, A., Haraszti, R. A., Harrison, P., Hegyesi, H., Hendrix, A., Hill, A. F., Hochberg, F. H., Hoffmann, K. F., Holder, B., Holthofer, H., Hosseinkhani, B., Hu, G., Huang, Y., Huber, V., Hunt, S., Ibrahim, A. G.‐E., Ikezu, T., Inal, J. M., Isin, M., Ivanova, A., Jackson, H. K., Jacobsen, S., Jay, S. M., Jayachandran, M., Jenster, G., Jiang, L., Johnson, S. M., Jones, J. C., Jong, A., Jovanovic‐Talisman, T., Jung, S., Kalluri, R., Kano, S. I., Kaur, S., Kawamura, Y., Keller, E. T., Khamari, D., Khomyakova, E., Khvorova, A., Kierulf, P., Kim, K. P., Kislinger, T., Klingeborn, M., Klinke, D. J., 2nd., Kornek, M., Kosanović, M. M., Kovács, Á. F., Krämer‐Albers, E.‐M., Krasemann, S., Krause, M., Kurochkin, I. V., Kusuma, G. D., Kuypers, S., Laitinen, S., Langevin, S. M., Languino, L. R., Lannigan, J., Lässer, C., Laurent, L. C., Lavieu, G., Lázaro‐Ibáñez, E., Le Lay, S., Lee, M.‐S., Lee, Y. X. F., Lemos, D. S., Lenassi, M., Leszczynska, A., Li, I. T., Liao, K., Libregts, S. F., Ligeti, E., Lim, R., Lim, S. K., Linē, A., Linnemannstöns, K., Llorente, A., Lombard, C. A., Lorenowicz, M. J., Lörincz, Á. M., Lötvall, J., Lovett, J., Lowry, M. C., Loyer, X., Lu, Q., Lukomska, B., Lunavat, T. R., Maas, S. L. N., Malhi, H., Marcilla, A., Mariani, J., Mariscal, J., Martens‐Uzunova, E. S., Martin‐Jaular, L., Martinez, M. C., Martins, V. R., Mathieu, M., Mathivanan, S., Maugeri, M., McGinnis, L. K., McVey, M. J., Meckes, D. G., Jr., Meehan, K. L., Mertens, I., Minciacchi, V. R., Möller, A., Møller Jørgensen, M., Morales‐Kastresana, A., Morhayim, J., Mullier, F., Muraca, M., Musante, L., Mussack, V., Muth, D. C., Myburgh, K. H., Najrana, T., Nawaz, M., Nazarenko, I., Nejsum, P., Neri, C., Neri, T., Nieuwland, R., Nimrichter, L., Nolan, J. P., Nolte‐’t Hoen, E. N. M., Hooten, N. N., O’Driscoll, L., O’Grady, T., O’Loghlen, A., Ochiya, T., Olivier, M., Ortiz, A., Ortiz, L. A., Osteikoetxea, X., Østergaard, O., Ostrowski, M., Park, J., Pegtel, D. M., Peinado, H., Perut, F., Pfaffl, M. W., Phinney, D. G., Pieters, B. C. H., Pink, R. C., Pisetsky, D. S., von Strandmann, E. P., Polakovicova, I., Poon, I. K. H., Powell, B. H., Prada, I., Pulliam, L., Quesenberry, P., Radeghieri, A., Raffai, R. L., Raimondo, S., Rak, J., Ramirez, M. I., Raposo, G., Rayyan, M. S., Regev‐Rudzki, N., Ricklefs, F. L., Robbins, P. D., Roberts, D. D., Rodrigues, S. C., Rohde, E., Rome, S., Rouschop, K. M. A., Rughetti, A., Russell, A. E., Saá, P., Sahoo, S., Salas‐Huenuleo, E., Sánchez, C., Saugstad, J. A., Saul, M. J., Schiffelers, R. M., Schneider, R., Schøyen, T. H., Scott, A., Shahaj, E., Sharma, S., Shatnyeva, O., Shekari, F., Shelke, G. V., Shetty, A. K., Shiba, K., Siljander, P. R.‐M., Silva, A. M., Skowronek, A., Snyder, O. L., 2nd., Soares, R. P., Sódar, B. W., Soekmadji, C., Sotillo, J., Stahl, P. D., Stoorvogel, W., Stott, S. L., Strasser, E. F., Swift, S., Tahara, H., Tewari, M., Timms, K., Tiwari, S., Tixeira, R., Tkach, M., Toh, W. S., Tomasini, R., Torrecilhas, A. C., Tosar, J. P., Toxavidis, V., Urbanelli, L., Vader, P., van Balkom, B. W. M., van der Grein, S. G., Van Deun, J., van Herwijnen, M. J. C., Van Keuren‐Jensen, K., van Niel, G., van Royen, M. E., van Wijnen, A. J., Vasconcelos, M. H., Vechetti, I. J., Jr., Veit, T. D., Vella, L. J., Velot, É., Verweij, F. J., Vestad, B., Viñas, J. L., Visnovitz, T., Vukman, K. V., Wahlgren, J., Watson, D. C., Wauben, M. H. M., Weaver, A., Webber, J. P., Weber, V., Wehman, A. M., Weiss, D. J., Welsh, J. A., Wendt, S., Wheelock, A. M., Wiener, Z., Witte, L., Wolfram, J., Xagorari, A., Xander, P., Xu, J., Yan, X., Yáñez‐Mó, M., Yin, H., Yuana, Y., Zappulli, V., Zarubova, J., Žėkas, V., Zhang, J.‐y., Zhao, Z., Zheng, L., Zheutlin, A. R., Zickler, A. M., Zimmermann, P., Zivkovic, A. M., Zocco, D., Zuba‐Surma, E. K., J. Extracell. Vesicles 2018, 7, 1535750.
dc.identifier.citedreferenceTauro, B. J., Greening, D. W., Mathias, R. A., Ji, H., Mathivanan, S., Scott, A. M., Simpson, R. J., Methods 2012, 56, 293 – 304.
dc.identifier.citedreferencePiersma, S. R., Fiedler, U., Span, S., Lingnau, A., Pham, T. V., Hoffmann, S., Kubbutat, M. H., Jiménez, C. R., J. Proteome Res. 2010, 9, 1913 – 1922.
dc.identifier.citedreferenceAnderson, N. L., Anderson, N. G., Mol. Cell. Proteomics 2002, 1, 845 – 867.
dc.identifier.citedreferenceTirumalai, R. S., Chan, K. C., Prieto, D. A., Issaq, H. J., Conrads, T. P., Veenstra, T. D., Mol. Cell. Proteomics 2003, 2, 1096 – 1103.
dc.identifier.citedreferenceAn, M., Wu, J., Zhu, J., Lubman, D. M., J. Proteome Res. 2018, 17, 3599 – 3605.
dc.identifier.citedreferenceChen, Y., Xie, Y., Xu, L., Zhan, S., Xiao, Y., Gao, Y., Wu, B., Ge, W., Int. J. Cancer 2017, 140, 900 – 913.
dc.identifier.citedreferenceBrennan, K., Martin, K., FitzGerald, S. P., O’Sullivan, J., Wu, Y., Blanco, A., Richardson, C., Mc Gee, M. M., Sci. Rep. 2020, 10, 1039.
dc.identifier.citedreferenceKarimi, N., Cvjetkovic, A., Jang, S. C., Crescitelli, R., Hosseinpour Feizi, M. A., Nieuwland, R., Lotvall, J., Lasser, C., Cell. Mol. Life Sci. 2018, 75, 2873 – 2886.
dc.identifier.citedreferenceWorsfold, P., Townshend, A., Poole, C. F., Miró, M., Encyclopedia of Analytical Science, 3rd edition, Elsevier, Amsterdam 2019.
dc.identifier.citedreferenceYanez‐Mo, M., Siljander, P. R., Andreu, Z., Zavec, A. B., Borras, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., Colas, E., Cordeiro‐da Silva, A., Fais, S., Falcon‐Perez, J. M., Ghobrial, I. M., Giebel, B., Gimona, M., Graner, M., Gursel, I., Gursel, M., Heegaard, N. H., Hendrix, A., Kierulf, P., Kokubun, K., Kosanovic, M., Kralj‐Iglic, V., Kramer‐Albers, E. M., Laitinen, S., Lasser, C., Lener, T., Ligeti, E., Line, A., Lipps, G., Llorente, A., Lotvall, J., Mancek‐Keber, M., Marcilla, A., Mittelbrunn, M., Nazarenko, I., Nolte‐’t Hoen, E. N., Nyman, T. A., O’Driscoll, L., Olivan, M., Oliveira, C., Pallinger, E., Del Portillo, H. A., Reventos, J., Rigau, M., Rohde, E., Sammar, M., Sanchez‐Madrid, F., Santarem, N., Schallmoser, K., Ostenfeld, M. S., Stoorvogel, W., Stukelj, R., Van der Grein, S. G., Vasconcelos, M. H., Wauben, M. H., De Wever, O., J. Extracell. Vesicles 2015, 4, 27066.
dc.identifier.citedreferenceLasser, C., Expert Opin. Biol. Ther. 2015, 15, 103 – 117.
dc.identifier.citedreferenceValadi, H., Ekstrom, K., Bossios, A., Sjostrand, M., Lee, J. J., Lotvall, J. O., Nat. Cell Biol. 2007, 9, 654 – 659.
dc.identifier.citedreferenceRaposo, G., Stoorvogel, W., J. Cell Biol. 2013, 200, 373 – 383.
dc.identifier.citedreferenceLazaro‐Ibanez, E., Sanz‐Garcia, A., Visakorpi, T., Escobedo‐Lucea, C., Siljander, P., Ayuso‐Sacido, A., Yliperttula, M., Prostate 2014, 74, 1379 – 1390.
dc.identifier.citedreferenceZhang, J., Li, S., Li, L., Li, M., Guo, C., Yao, J., Mi, S., Genomics, Proteomics Bioinf. 2015, 13, 17 – 24.
dc.identifier.citedreferenceChen, I. H., Xue, L., Hsu, C. C., Paez, J. S., Pan, L., Andaluz, H., Wendt, M. K., Iliuk, A. B., Zhu, J. K., Tao, W. A., Proc. Natl. Acad. Sci. USA 2017, 114, 3175 – 3180.
dc.identifier.citedreferenceMelo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., Reissfelder, C., Pilarsky, C., Fraga, M. F., Piwnica‐Worms, D., Kalluri, R., Nature 2015, 523, 177 – 182.
dc.identifier.citedreferenceZhang, W., Xia, W., Lv, Z., Ni, C., Xin, Y., Yang, L., Cell. Physiol. Biochem. 2017, 41, 755 – 768.
dc.identifier.citedreferenceKhan, M., Nickoloff, E., Abramova, T., Johnson, J., Verma, S. K., Krishnamurthy, P., Mackie, A. R., Vaughan, E., Garikipati, V. N., Benedict, C., Ramirez, V., Lambers, E., Ito, A., Gao, E., Misener, S., Luongo, T., Elrod, J., Qin, G., Houser, S. R., Koch, W. J., Kishore, R., Circ. Res. 2015, 117, 52 – 64.
dc.identifier.citedreferenceArbelaiz, A., Azkargorta, M., Krawczyk, M., Santos‐Laso, A., Lapitz, A., Perugorria, M. J., Erice, O., Gonzalez, E., Jimenez‐Aguero, R., Lacasta, A., Ibarra, C., Sanchez‐Campos, A., Jimeno, J. P., Lammert, F., Milkiewicz, P., Marzioni, M., Macias, R. I. R., Marin, J. J. G., Patel, T., Gores, G. J., Martinez, I., Elortza, F., Falcon‐Perez, J. M., Bujanda, L., Banales, J. M., Hepatology 2017, 66, 1125 – 1143.
dc.identifier.citedreferenceWang, N., Song, X., Liu, L., Niu, L., Wang, X., Song, X., Xie, L., Cancer Sci. 2018, 109, 1701 – 1709.
dc.identifier.citedreferenceAbramowicz, A., Widlak, P., Pietrowska, M., Mol. BioSyst. 2016, 12, 1407 – 1419.
dc.identifier.citedreferenceKeller, B. O., Sui, J., Young, A. B., Whittal, R. M., Anal. Chim. Acta 2008, 627, 71 – 81.
dc.identifier.citedreferenceAn, M., Lohse, I., Tan, Z., Zhu, J., Wu, J., Kurapati, H., Morgan, M. A., Lawrence, T. S., Cuneo, K. C., Lubman, D. M., J. Proteome Res. 2017, 16, 1763 – 1772.
dc.working.doi10.7302/194en
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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