A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage- derived IncY plasmid
Kamal, Shady Mansour; Cimdins‐ahne, Annika; Lee, Changhan; Li, Fengyang; Martín‐rodríguez, Alberto J.; Seferbekova, Zaira; Afasizhev, Robert; Wami, Haleluya Tesfaye; Katikaridis, Panagiotis; Meins, Lena; Lünsdorf, Heinrich; Dobrindt, Ulrich; Mogk, Axel; Römling, Ute
2021-02
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Kamal, Shady Mansour; Cimdins‐ahne, Annika ; Lee, Changhan; Li, Fengyang; Martín‐rodríguez, Alberto J. ; Seferbekova, Zaira; Afasizhev, Robert; Wami, Haleluya Tesfaye; Katikaridis, Panagiotis; Meins, Lena; Lünsdorf, Heinrich ; Dobrindt, Ulrich; Mogk, Axel; Römling, Ute (2021). "A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage- derived IncY plasmid." Molecular Microbiology 115(2): 255-271.
Abstract
The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K- 12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K- 12, the 4.88 Mbp Fec10 genome is characterized by distinct single- nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two- component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand- alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI- Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI- SG17M. Furthermore, we noted a unique contribution of ClpGGI- Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food- borne heat- resistant strains in the human gut.The ubiquitousness of commensal Escherichia coli in post- industrial humans calls for the investigation of their physiological characteristics. Thermotolerance, unconventional anaerobic respiration, and detoxification cargo genes are encoded on an IncY derived plasmid harbored by a typical member of the common ST10 clone. Thermotolerance is well represented in commensal isolates with the key disaggregase ClpGGI to be exquisitely thermostable. Fire symbol by ya- webdesign.com.Publisher
Wiley Periodicals, Inc.
ISSN
0950-382X 1365-2958
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