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Automated leukocyte parameters are useful in the assessment of myelodysplastic syndromes
dc.contributor.authorShestakova, Anna
dc.contributor.authorNael, Ali
dc.contributor.authorNora, Virgilita
dc.contributor.authorRezk, Sherif
dc.contributor.authorZhao, Xiaohui
dc.date.accessioned2021-06-02T21:10:16Z
dc.date.available2022-06-02 17:10:14en
dc.date.available2021-06-02T21:10:16Z
dc.date.issued2021-05
dc.identifier.citationShestakova, Anna; Nael, Ali; Nora, Virgilita; Rezk, Sherif; Zhao, Xiaohui (2021). "Automated leukocyte parameters are useful in the assessment of myelodysplastic syndromes." Cytometry Part B: Clinical Cytometry 100(3): 299-311.
dc.identifier.issn1552-4949
dc.identifier.issn1552-4957
dc.identifier.urihttps://hdl.handle.net/2027.42/167851
dc.description.abstractBackgroundStudy utility of seven automated VCS parameters (V‐volume, C‐conductivity and S‐scatter) in leukocytes as an objective read‐out of dysplasia in Myelodysplastic Syndromes (MDS).MethodsPeripheral blood was analyzed by Beckman‐Coulter DxH800 hematology analyzer in 43 patients with low‐grade, high‐grade MDS and 21 control individuals. The differences in mean (MN) and standard deviation (SD) of each parameter were examined. The optimal sensitivity and specificity to predict MDS were determined by statistical analysis.ResultsIn neutrophils, all means of the light scatters were significantly lower in high‐grade MDS than in the control group. Mean median angle light scatter (MN‐MALS‐NE) and mean upper median angle light scatter (MN‐UMALS‐NE) were significantly different between low‐grade MDS and control patients. MN‐MALS‐NE as a MDS predictor revealed 63% sensitivity and 67% specificity with a cutoff value of ≤133. SDs of each parameter in neutrophils differed significantly among three groups. SD of neutrophil upper median angle light scatter (SD‐UMALS‐NE) had 77% sensitivity and 82% specificity (cutoff value of ≥11.16) to predict MDS.ConclusionsMDS patients have a significant decrease with a linear trend in VCS parameters in neutrophils, indicating cell dysplasia. The degree of the heterogeneity measured by SD is the most predictive of MDS.
dc.publisherJohn Wiley & Sons, Inc.
dc.subject.otherlight scatter parameters 3
dc.subject.othermyelodysplastic syndromes 1
dc.subject.otherautomatic hematology analyzer 2
dc.titleAutomated leukocyte parameters are useful in the assessment of myelodysplastic syndromes
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMedicine (General)
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/167851/1/cytob21947.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/167851/2/cytob21947_am.pdf
dc.identifier.doi10.1002/cyto.b.21947
dc.identifier.sourceCytometry Part B: Clinical Cytometry
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dc.working.doiNOen
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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