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Isolation of Circulating Biomarkers for Liquid Biopsy using Immunoaffinity- Based Stimuli- Responsive Hybrid Hydrogel Beads
dc.contributor.authorKang, Yoon‐tae
dc.contributor.authorKim, Young Jun
dc.contributor.authorRupp, Brittany
dc.contributor.authorPurcell, Emma
dc.contributor.authorHadlock, Thomas
dc.contributor.authorRamnath, Nithya
dc.contributor.authorNagrath, Sunitha
dc.date.accessioned2021-08-03T18:16:23Z
dc.date.available2022-07-03 14:16:22en
dc.date.available2021-08-03T18:16:23Z
dc.date.issued2021-06
dc.identifier.citationKang, Yoon‐tae ; Kim, Young Jun; Rupp, Brittany; Purcell, Emma; Hadlock, Thomas; Ramnath, Nithya; Nagrath, Sunitha (2021). "Isolation of Circulating Biomarkers for Liquid Biopsy using Immunoaffinity- Based Stimuli- Responsive Hybrid Hydrogel Beads." Analysis & Sensing 1(3): 117-129.
dc.identifier.issn2629-2742
dc.identifier.issn2629-2742
dc.identifier.urihttps://hdl.handle.net/2027.42/168497
dc.description.abstractThis work presents chemically stable and biodegradable hydrogel beads for the isolation of circulating tumor cells (CTCs) and circulating exosomes in liquid biopsy. The liquid biopsy hydrogel beads (LBbeads) consisting of alginate and poly(vinyl alcohol) hydrogels show both chemical stability and stimuli- degradable characteristics. Unlike single- component hydrogels, this hybrid form is not easily degraded by buffers or cell culture media while its degradable characteristic remains; thus, it is useful in bio- applications requiring multi- step processes with various reagents and lengthy incubation periods. We applied our platform to clinical samples for isolating two promising circulating biomarkers for a liquid biopsy, CTCs and exosomes, by conjugating the hydrogel surface with anti- EpCAM and anti- CD63 antibodies, respectively, thus achieving 37.4 CTCs and comparable amount of exosome recovery per 1 milliliter of blood. The results show easy device- free isolation and retrieval of CTCs and exosomes, with recovered circulating biomarkers successfully analyzed by western blot analysis and fluorescence microscopy. We believe that this simple and versatile platform enables us to isolate prominent circulating biomarkers for clinical use in cancer diagnosis.Circulating markers isolation using hydrogel- beads: this work reports a hybrid hydrogel platform facilitating isolation of two promising liquid biopsy biomarkers, circulating tumor cells and circulating exosomes. The results show easy device- free isolation and retrieval of CTCs and exosomes, with recovered circulating biomarkers successfully analyzed by western blot analysis and fluorescence microscopy. This simple and versatile platform makes it possible to isolate prominent circulating biomarkers for clinical use in cancer diagnosis.
dc.publisherWiley Periodicals, Inc.
dc.subject.othercirculating tumor cells
dc.subject.otherhydrogels
dc.subject.otherantibodies
dc.subject.otherexosomes
dc.subject.otherliquid biopsy
dc.titleIsolation of Circulating Biomarkers for Liquid Biopsy using Immunoaffinity- Based Stimuli- Responsive Hybrid Hydrogel Beads
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/168497/1/anse202100016_am.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/168497/2/anse202100016-sup-0001-misc_information.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/168497/3/anse202100016.pdf
dc.identifier.doi10.1002/anse.202100016
dc.identifier.sourceAnalysis & Sensing
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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