Effect of red wine or its polyphenols on induced apical periodontitis in rats
Dal‐fabbro, Renan; Cosme‐Silva, Leopoldo; Rezende Silva Martins de Oliveira, Fernanda; Capalbo, Letícia Cabrera; Plazza, Flávia Alfredo; Ervolino, Edilson; Cintra, Luciano Tavares Angelo; Gomes‐Filho, João Eduardo
2021-12
Citation
Dal‐fabbro, Renan ; Cosme‐Silva, Leopoldo ; Rezende Silva Martins de Oliveira, Fernanda; Capalbo, Letícia Cabrera ; Plazza, Flávia Alfredo ; Ervolino, Edilson; Cintra, Luciano Tavares Angelo; Gomes‐Filho, João Eduardo (2021). "Effect of red wine or its polyphenols on induced apical periodontitis in rats." International Endodontic Journal (12): 2276-2289.
Abstract
AimTo evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with apical periodontitis in rats.MethodologyThirty- two three- month- old Wistar rats had apical periodontitis induced in four first molars and were then arranged into four groups: control (C)- rats with apical periodontitis; wine (W)- rats with apical periodontitis receiving 4.28 ml/kg of red wine; resveratrol+quercetin (R+Q)- rats with apical periodontitis receiving 4.28 ml/kg of a solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol and alcohol (ALC)- rats with apical periodontitis receiving the alcoholic dose contained in the wine. The oral gavage treatments were administered daily, from day 0 to day 45. On the 15th day, apical periodontitis was induced, and on the 45th day, the animals were euthanized. Histological, immunohistochemical (RANKL, OPG, TRAP, IL- 10, TNF- - º and IL- 1β) and micro- computed tomography for bone resorption analysis were performed in the jaws. The Kruskal- Wallis with Dunn’s test was performed for nonparametric data, and the anova with Tukey’s test for parametric data, p < .05.ResultsThe median score of the inflammatory process was significantly lower in the R+Q group (1) compared to the C (2) (p = .0305) and ALC (3) (p = .0003) groups, and not different from the W (1.5) group. The immunolabeling for OPG was significantly higher in the R+Q group (p = .0054) compared to all groups; the same was observed for IL- 10 (p = .0185), different from groups C and ALC. The R+Q group had the lowest TRAP cell count (p < .0001), followed by the W group, both inferior to C and ALC groups. The lowest bone resorption value was in the R+Q group (0.50mm3 ± 0.21mm3), significantly lower (p = .0292) than the C group (0.88mm3 ± 0.10mm3). The W group (0.60 mm3 ± 0.25 mm3) and R+Q group had less bone resorption compared to the ALC group (0.97 mm3 ± 0.22 mm3), p = .0297 and p = .0042, respectively.ConclusionRed wine administration to rats for 15 days before induction of apical periodontitis decreased inflammation, TRAP marking and periapical bone resorption compared to alcohol. Resveratrol- quercetin administration reduced the inflammatory process in apical periodontitis, periapical bone resorption, and altered the OPG, IL- 10 and TRAP expression compared to C and ALC groups.Publisher
Wiley Periodicals, Inc.
ISSN
0143-2885 1365-2591
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