View Item 

Show simple item record

The holy basil administration diminishes the NF-kB expression and protects alveolar epithelial cells from pneumonia infection through interferon gamma
dc.contributor.authorSuresh, Arundhathy
dc.contributor.authorRao, Tejeshwar C.
dc.contributor.authorSolanki, Sumeet
dc.contributor.authorSuresh, Madathilparambil V.
dc.contributor.authorMenon, Bindu
dc.contributor.authorRaghavendran, Krishnan
dc.date.accessioned2022-05-06T17:29:28Z
dc.date.available2023-05-06 13:29:26en
dc.date.available2022-05-06T17:29:28Z
dc.date.issued2022-04
dc.identifier.citationSuresh, Arundhathy; Rao, Tejeshwar C.; Solanki, Sumeet; Suresh, Madathilparambil V.; Menon, Bindu; Raghavendran, Krishnan (2022). "The holy basil administration diminishes the NF-kB expression and protects alveolar epithelial cells from pneumonia infection through interferon gamma." Phytotherapy Research 36(4): 1822-1835.
dc.identifier.issn0951-418X
dc.identifier.issn1099-1573
dc.identifier.urihttps://hdl.handle.net/2027.42/172333
dc.description.abstractBacterial pneumonia is one of the most important causes of mortality in the United States. The bacteria Klebsiella pneumoniae (KP) accounts for a significant proportion of community and hospital-acquired infections. Here, we determine that the holy basil (Ocimum sanctum) extract improves cell viability and dampens the proinflammatory cytokine response in an in vitro model of pneumonia. For this, A549, a human alveolar basal epithelial cell line, was subjected to a lethal KP model following a 24-hr pretreatment with basil extract. Bacteremia, cell viability, apoptosis, MTT assay, phagocytic capacity, cytokines, and Khe gene expression were assessed in these cells following pneumonia. Cell morphology analysis showed that holy basil protected A549 cells from KP infection–mediated effects by inhibiting cell death due to apoptosis. Additionally, in the presence of basil, A549 cells demonstrated significantly higher bactericidal capacity and phagocytosis. Administration of holy basil led to reduced expression of hypoxia-inducible factor-1/2a, nuclear factor kappa B, and Khe in the KP-infected cells while increasing interferon (IFN)-γ expression. Our results suggest that basil significantly reduced cell death in the setting of KP infection, likely via attenuation of cytokine and IFN-γ mediated signaling pathways. Holy basil is a promising therapeutic agent for managing and treating bacterial pneumonia based on its potency.
dc.publisherJohn Wiley & Sons, Ltd.
dc.subject.otherphagocytosis
dc.subject.otherpneumonia
dc.subject.otherhuman lung epithelial cells
dc.subject.otherapoptosis
dc.subject.otherholy basil
dc.titleThe holy basil administration diminishes the NF-kB expression and protects alveolar epithelial cells from pneumonia infection through interferon gamma
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/172333/1/ptr7428_am.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/172333/2/ptr7428.pdf
dc.identifier.doi10.1002/ptr.7428
dc.identifier.sourcePhytotherapy Research
dc.identifier.citedreferenceSuresh, M. V., Dolgachev, V. A., Zhang, B., Balijepalli, S., Swamy, S., Mooliyil, J., … Lalwani, S. ( 2019 ). TLR3 absence confers increased survival with improved macrophage activity against pneumonia. JCI Insight, 4 ( 23 ), e131195. https://doi.org/10.1172/jci.insight.131195
dc.identifier.citedreferenceRijneveld, A. W., Lauw, F. N., Schultz, M. J., Florquin, S., Te Velde, A. A., Speelman, P., … Van Der Poll, T. ( 2002 ). The role of interferon-gamma in murine pneumococcal pneumonia. The Journal of Infectious Diseases, 185 ( 1 ), 91 – 97. https://doi.org/10.1086/338122
dc.identifier.citedreferenceSaharkhiz, M. J., Kamyab, A. A., Kazerani, N. K., Zomorodian, K., Pakshir, K., & Rahimi, M. J. ( 2015 ). Chemical compositions and antimicrobial activities of Ocimum sanctum L. essential oils at different harvest stages. Jundishapur Journal of Microbiology, 8 ( 1 ), e13720. https://doi.org/10.5812/jjm.13720
dc.identifier.citedreferenceShetty, S., Udupa, S., & Udupa, L. ( 2008 ). Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evidence-Based Complementary and Alternative Medicine, 5 ( 1 ), 95 – 101. https://doi.org/10.1093/ecam/nem004
dc.identifier.citedreferenceShivananjappa, M. M., Mhasavade, D., & Joshi, M. K. ( 2013 ). Aqueous extract of Terminalia arjuna attenuates tert-butyl hydroperoxide-induced oxidative stress in HepG2 cell model. Cell Biochemistry and Function, 31 ( 2 ), 129 – 135. https://doi.org/10.1002/cbf.2867
dc.identifier.citedreferenceSimamura, E., Hirai, K. I., Shimada, H., & Koyama, J. ( 2001 ). Apoptosis and epithelial phagocytosis in mitomycin C-treated human pulmonary adenocarcinoma A549 cells. Tissue & Cell, 33 ( 2 ), 161 – 168.
dc.identifier.citedreferenceSingh, S., Rehan, H. M., & Majumdar, D. K. ( 2001 ). Effect of Ocimum sanctum fixed oil on blood pressure, blood clotting time and pentobarbitone-induced sleeping time. Journal of Ethnopharmacology, 78 ( 2–3 ), 139 – 143.
dc.identifier.citedreferenceStandiford, T. J., & Huffnagle, G. B. ( 1997 ). Cytokines in host defense against pneumonia. Journal of Investigative Medicine, 45 ( 6 ), 335 – 345.
dc.identifier.citedreferenceStrieter, R. M., & Kunkel, S. L. ( 1994 ). Acute lung injury: The role of cytokines in the elicitation of neutrophils. Journal of Investigative Medicine, 42 ( 4 ), 640 – 651.
dc.identifier.citedreferenceStrieter, R. M., Kunkel, S. M., Keane, M. P., & Standiford, T. J. ( 1999 ). Chemokines in lung injury: Thomas A. Neff lecture. Chest, 116 ( 1 ), 103S – 110S.
dc.identifier.citedreferenceSuresh, M. V., Balijepalli, S., Zhang, B., Singh, V. V., Swamy, S., Panicker, S., … Rao, T. C. ( 2019 ). Hypoxia-inducible factor (HIF)-1alpha promotes inflammation and injury following aspiration-induced lung injury in mice. Shock, 52 ( 6 ), 612 – 621. https://doi.org/10.1097/SHK.0000000000001312
dc.identifier.citedreferenceSuresh, M. V., Thomas, B., Machado-Aranda, D., Dolgachev, V. A., Kumar Ramakrishnan, S., Talarico, N., … Walter, N. G. ( 2016 ). Double-stranded RNA interacts with toll-like receptor 3 in driving the acute inflammatory response following lung contusion. Critical Care Medicine, 44 ( 11 ), e1054 – e1066. https://doi.org/10.1097/CCM.0000000000001879
dc.identifier.citedreferenceSuresh, M. V., Wagner, M. C., Rosania, G. R., Stringer, K. A., Min, K. A., Risler, L., … Reddy, R. C. ( 2012 ). Pulmonary administration of a water-soluble curcumin complex reduces severity of acute lung injury. American Journal of Respiratory Cell and Molecular Biology, 47 ( 3 ), 280 – 287. https://doi.org/10.1165/rcmb.2011-0175OC
dc.identifier.citedreferenceSuresh, M. V., Yu, B., Lakshminrusimha, S., Machado-Aranda, D., Talarico, N., Zeng, L., … Raghavendran, K. ( 2013 ). The protective role of MnTBAP in oxidant-mediated injury and inflammation in a rat model of lung contusion. Surgery, 154 ( 5 ), 980 – 990. https://doi.org/10.1016/j.surg.2013.05.023 S0039-6060(13)00268-7 [pii].
dc.identifier.citedreferenceTaylor, M., Qu, A., Anderson, E. R., Matsubara, T., Martin, A., Gonzalez, F. J., & Shah, Y. M. ( 2011 ). Hypoxia-inducible factor-2alpha mediates the adaptive increase of intestinal ferroportin during iron deficiency in mice. Gastroenterology, 140 ( 7 ), 2044 – 2055. https://doi.org/10.1053/j.gastro.2011.03.007
dc.identifier.citedreferenceUma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K. K., & Unnikrishnan, M. K. ( 2000 ). Radiation protection by the ocimum flavonoids orientin and vicenin: Mechanisms of action. Radiation Research, 154 ( 4 ), 455 – 460.
dc.identifier.citedreferencevan Uden, P., Kenneth, N. S., & Rocha, S. ( 2008 ). Regulation of hypoxia-inducible factor-1alpha by NF-kappaB. The Biochemical Journal, 412 ( 3 ), 477 – 484. https://doi.org/10.1042/BJ20080476
dc.identifier.citedreferenceVeluthakal, R., Suresh, M. V., & Kowluru, A. ( 2009 ). Down-regulation of expression and function of nucleoside diphosphate kinase in insulin-secreting beta-cells under in vitro conditions of glucolipotoxicity. Molecular and Cellular Biochemistry, 329 ( 1–2 ), 121 – 129. https://doi.org/10.1007/s11010-009-0113-6
dc.identifier.citedreferenceWalley, K. R., Lukacs, N. W., Standiford, T. J., Strieter, R. M., & Kunkel, S. L. ( 1996 ). Balance of inflammatory cytokines related to severity and mortality of murine sepsis. Infection and Immunity, 64 ( 11 ), 4733 – 4738.
dc.identifier.citedreferenceWang, E., Ouellet, N., Simard, M., Fillion, I., Bergeron, Y., Beauchamp, D., & Bergeron, M. G. ( 2001 ). Pulmonary and systemic host response to Streptococcus pneumoniae and Klebsiella pneumoniae bacteremia in normal and immunosuppressed mice. Infection and Immunity, 69 ( 9 ), 5294 – 5304.
dc.identifier.citedreferenceWang, G. L., Jiang, B. H., Rue, E. A., & Semenza, G. L. ( 1995 ). Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O 2 tension. Proceedings of the National Academy of Sciences of the United States of America, 92 ( 12 ), 5510 – 5514.
dc.identifier.citedreferenceWang, G. L., & Semenza, G. L. ( 1993 ). Characterization of hypoxia-inducible factor 1 and regulation of DNA binding activity by hypoxia. The Journal of Biological Chemistry, 268 ( 29 ), 21513 – 21518.
dc.identifier.citedreferenceWen, H., Dou, Y., Hogaboam, C. M., & Kunkel, S. L. ( 2008 ). Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response. Blood, 111 ( 4 ), 1797 – 1804. https://doi.org/10.1182/blood-2007-08-106443
dc.identifier.citedreferenceWhite, R. J., Blainey, A. D., Harrison, K. J., & Clarke, S. K. ( 1981 ). Causes of pneumonia presenting to a district general hospital. Thorax, 36 ( 8 ), 566 – 570.
dc.identifier.citedreferenceWinter, C., Taut, K., Srivastava, M., Langer, F., Mack, M., Briles, D. E., … Maus, U. A. ( 2007 ). Lung-specific overexpression of CC chemokine ligand (CCL) 2 enhances the host defense to Streptococcus pneumoniae infection in mice: Role of the CCL2-CCR2 axis. Journal of Immunology, 178 ( 9 ), 5828 – 5838 178/9/5828 [pii].
dc.identifier.citedreferenceYoshizaki, K., Nishimoto, N., Matsumoto, K., Tagoh, H., Taga, T., Deguchi, Y., … Kishimoto, T. ( 1990 ). Interleukin 6 and expression of its receptor on epidermal keratinocytes. Cytokine, 2, 381 – 387.
dc.identifier.citedreferenceZeng, X., Moore, T. A., Newstead, M. W., Deng, J. C., Kunkel, S. L., Luster, A. D., & Standiford, T. J. ( 2005 ). Interferon-inducible protein 10, but not monokine induced by gamma interferon, promotes protective type 1 immunity in murine Klebsiella pneumoniae pneumonia. Infection and Immunity, 73 ( 12 ), 8226 – 8236. https://doi.org/10.1128/IAI.73.12.8226-8236.2005 73/12/8226 [pii].
dc.identifier.citedreferenceZhang, B., Swamy, S., Balijepalli, S., Panicker, S., Mooliyil, J., Sherman, M. A., … Suresh, M. V. ( 2019 ). Direct pulmonary delivery of solubilized curcumin reduces severity of lethal pneumonia. The FASEB Journal, 33 ( 12 ), 13294 – 13309. https://doi.org/10.1096/fj.201901047RR
dc.identifier.citedreferenceAhmad, A., Abuzinadah, M. F., Alkreathy, H. M., Banaganapalli, B., & Mujeeb, M. ( 2018 ). Ursolic acid rich Ocimum sanctum L leaf extract loaded nanostructured lipid carriers ameliorate adjuvant induced arthritis in rats by inhibition of COX-1, COX-2, TNF-alpha and IL-1: Pharmacological and docking studies. PLoS One, 13 ( 3 ), e0193451. https://doi.org/10.1371/journal.pone.0193451
dc.identifier.citedreferenceBaliga, M. S., Jimmy, R., Thilakchand, K. R., Sunitha, V., Bhat, N. R., Saldanha, E., … Palatty, P. L. ( 2013 ). Ocimum sanctum L (holy basil or Tulsi) and its phytochemicals in the prevention and treatment of cancer. Nutrition and Cancer, 65 ( Suppl 1 ), 26 – 35. https://doi.org/10.1080/01635581.2013.785010
dc.identifier.citedreferenceBallinger, M. N., Paine, R., 3rd, Serezani, C. H., Aronoff, D. M., Choi, E. S., Standiford, T. J., … Moore, B. B. ( 2006 ). Role of granulocyte macrophage colony-stimulating factor during gram-negative lung infection with Pseudomonas aeruginosa. American Journal of Respiratory Cell and Molecular Biology, 34 ( 6 ), 766 – 774. https://doi.org/10.1165/rcmb.2005-0246OC
dc.identifier.citedreferenceBeekman, C., Janson, A. A., Baghat, A., van Deutekom, J. C., & Datson, N. A. ( 2018 ). Use of capillary Western immunoassay (Wes) for quantification of dystrophin levels in skeletal muscle of healthy controls and individuals with Becker and Duchenne muscular dystrophy. PLoS One, 13 ( 4 ), e0195850. https://doi.org/10.1371/journal.pone.0195850
dc.identifier.citedreferenceBroug-Holub, E., Toews, G. B., van Iwaarden, J. F., Strieter, R. M., Kunkel, S. L., Paine, R., 3rd, & Standiford, T. J. ( 1997 ). Alveolar macrophages are required for protective pulmonary defenses in murine Klebsiella pneumonia: Elimination of alveolar macrophages increases neutrophil recruitment but decreases bacterial clearance and survival. Infection and Immunity, 65 ( 4 ), 1139 – 1146.
dc.identifier.citedreferenceCai, S., Batra, S., Lira, S. A., Kolls, J. K., & Jeyaseelan, S. ( 2010 ). CXCL1 regulates pulmonary host defense to Klebsiella infection via CXCL2, CXCL5, NF-kappaB, and MAPKs. Journal of Immunology, 185 ( 10 ), 6214 – 6225. https://doi.org/10.4049/jimmunol.0903843
dc.identifier.citedreferenceCavalcanti, Y. V., Brelaz, M. C., Neves, J. K., Ferraz, J. C., & Pereira, V. R. ( 2012 ). Role of TNF-alpha, IFN-gamma, and IL-10 in the development of pulmonary tuberculosis. Pulmonary Medicine, 2012, 745483. https://doi.org/10.1155/2012/745483
dc.identifier.citedreferenceChen, Q., Huang, Y., Yang, Y., & Qiu, H. ( 2013 ). Acidinduced cell injury and death in lung epithelial cells is associated with the activation of mitogenactivated protein kinases. Molecular Medicine Reports, 8 ( 2 ), 565 – 570. https://doi.org/10.3892/mmr.2013.1537
dc.identifier.citedreferenceCilloniz, C., Martin-Loeches, I., Garcia-Vidal, C., San Jose, A., & Torres, A. ( 2016 ). Microbial etiology of pneumonia: Epidemiology, diagnosis and resistance patterns. International Journal of Molecular Sciences, 17 ( 12 ), 2120. https://doi.org/10.3390/ijms17122120
dc.identifier.citedreferenceCohen, M. M. ( 2014 ). Tulsi- Ocimum sanctum: A herb for all reasons. Journal of Ayurveda and Integrative Medicine, 5 ( 4 ), 251 – 259. https://doi.org/10.4103/0975-9476.146554
dc.identifier.citedreferenceCraven, D. E., Barber, T. W., Steger, K. A., & Montecalvo, M. A. ( 1990 ). Nosocomial pneumonia in the 1990s: Update of epidemiology and risk factors. Seminars in Respiratory Infections, 5, 157 – 172.
dc.identifier.citedreferenceDalton, D. K., Pitts-Meek, S., Keshav, S., Figari, I. S., Bradley, A., & Stewart, T. A. ( 1993 ). Multiple defects of immune cell function in mice with disrupted interferon-gamma genes. Science, 259 ( 5102 ), 1739 – 1742.
dc.identifier.citedreferencede Jager, W., Bourcier, K., Rijkers, G. T., Prakken, B. J., & Seyfert-Margolis, V. ( 2009 ). Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays. BMC Immunology, 10, 52. https://doi.org/10.1186/1471-2172-10-52
dc.identifier.citedreferenceDocke, W. D., Randow, F., Syrbe, U., Krausch, D., Asadullah, K., Reinke, P., … Kox, W. ( 1997 ). Monocyte deactivation in septic patients: Restoration by IFN-gamma treatment. Nature Medicine, 3, 678 – 681.
dc.identifier.citedreferenceDolgachev, V., Panicker, S., Balijepalli, S., McCandless, L. K., Yin, Y., Swamy, S., … Machado-Aranda, D. ( 2018 ). Electroporation-mediated delivery of FER gene enhances innate immune response and improves survival in a murine model of pneumonia. Gene Therapy, 25 ( 5 ), 359 – 375. https://doi.org/10.1038/s41434-018-0022-y
dc.identifier.citedreferenceDolgachev, V. A., Yu, B., Reinke, J. M., Raghavendran, K., & Hemmila, M. R. ( 2012 ). Host susceptibility to gram-negative pneumonia after lung contusion. Journal of Trauma and Acute Care Surgery, 72 ( 3 ), 614 – 622; discussion 622-613. [pii] 01586154–201203000-00010. https://doi.org/10.1097/TA.0b013e318243d9b1
dc.identifier.citedreferenceGautam, M. K., & Goel, R. K. ( 2014 ). Toxicological study of Ocimum sanctum Linn leaves: Hematological, biochemical, and histopathological studies. Journal of Toxicology, 2014, 135654. https://doi.org/10.1155/2014/135654
dc.identifier.citedreferenceGuo, J., & Cheng, Y. ( 2018 ). MicroRNA-1247 inhibits lipopolysaccharides-induced acute pneumonia in A549 cells via targeting CC chemokine ligand 16. Biomedicine & Pharmacotherapy, 104, 60 – 68. https://doi.org/10.1016/j.biopha.2018.05.012
dc.identifier.citedreferenceJamshidi, N., & Cohen, M. M. ( 2017 ). The clinical efficacy and safety of Tulsi in humans: A systematic review of the literature. Evidence-Based Complementary and Alternative Medicine, 2017, 9217567. https://doi.org/10.1155/2017/9217567
dc.identifier.citedreferenceKaiser, W. J., & Offermann, M. K. ( 2005 ). Apoptosis induced by the toll-like receptor adaptor TRIF is dependent on its receptor interacting protein homotypic interaction motif. Journal of Immunology, 174 ( 8 ), 4942 – 4952.
dc.identifier.citedreferenceKhan, A., Ahmad, A., Akhtar, F., Yousuf, S., Xess, I., Khan, L. A., & Manzoor, N. ( 2010 ). Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity. Research in Microbiology, 161 ( 10 ), 816 – 823. https://doi.org/10.1016/j.resmic.2010.09.008
dc.identifier.citedreferenceLeu, H. S., Kaiser, D. L., Mori, M., Woolson, R. F., & Wenzel, R. P. ( 1989 ). Hospital-acquired pneumonia. Attributable mortality and morbidity. American Journal of Epidemiology, 129 ( 6 ), 1258 – 1267.
dc.identifier.citedreferenceMartin, T. R., Hagimoto, N., Nakamura, M., & Matute-Bello, G. ( 2005 ). Apoptosis and epithelial injury in the lungs. Proceedings of the American Thoracic Society, 2 ( 3 ), 214 – 220. https://doi.org/10.1513/pats.200504-031AC 2/3/214 [pii].
dc.identifier.citedreferenceMiller, N. M., Wang, J., Tan, Y., & Dittel, B. N. ( 2015 ). Anti-inflammatory mechanisms of IFN-gamma studied in experimental autoimmune encephalomyelitis reveal neutrophils as a potential target in multiple sclerosis. Frontiers in Neuroscience, 9, 287. https://doi.org/10.3389/fnins.2015.00287
dc.identifier.citedreferenceMiller, P. R., Croce, M. A., Bee, T. K., Qaisi, W. G., Smith, C. P., Collins, G. L., & Fabian, T. C. ( 2001 ). ARDS after pulmonary contusion: Accurate measurement of contusion volume identifies high-risk patients. The Journal of Trauma, 51 ( 2 ), 223 – 228 discussion 229-230.
dc.identifier.citedreferenceMondal, S., Mirdha, B. R., & Mahapatra, S. C. ( 2009 ). The science behind sacredness of Tulsi ( Ocimum sanctum Linn.). Indian Journal of Physiology and Pharmacology, 53 ( 4 ), 291 – 306.
dc.identifier.citedreferenceNaji-Tabasi, S., Razavi, S. M., Mohebbi, M., & Malaekeh-Nikouei, B. ( 2016 ). New studies on basil ( Ocimum bacilicum L.) seed gum: Part I–Fractionation, physicochemical and surface activity characterization. Food Hydrocolloids, 52, 350 – 358.
dc.identifier.citedreferencePattanayak, P., Behera, P., Das, D., & Panda, S. K. ( 2010 ). Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy Reviews, 4 ( 7 ), 95 – 105. https://doi.org/10.4103/0973-7847.65323
dc.identifier.citedreferenceRaina, P., Chandrasekaran, C. V., Deepak, M., Agarwal, A., & Ruchika, K. G. ( 2015 ). Evaluation of subacute toxicity of methanolic/aqueous preparation of aerial parts of O. sanctum in Wistar rats: Clinical, haematological, biochemical and histopathological studies. Journal of Ethnopharmacology, 175, 509 – 517. https://doi.org/10.1016/j.jep.2015.10.015
dc.working.doiNOen
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
ptr7428_am.pdf
Size:
1.077MB
Format:
PDF
View/Open
Name:
ptr7428.pdf
Size:
2.945MB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.