Murine brain tumor microenvironment immunophenotyping using mass cytometry
dc.contributor.author | McClellan, BL | |
dc.contributor.author | Alghamri, MS | |
dc.contributor.author | Thalla, R | |
dc.contributor.author | Lowenstein, PR | |
dc.contributor.author | Castro, MG | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2022-09-15T18:53:46Z | |
dc.date.available | 2022-09-15T18:53:46Z | |
dc.date.issued | 2022-06-17 | |
dc.identifier.issn | 2666-1667 | |
dc.identifier.issn | 2666-1667 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/35634359 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/174690 | en |
dc.description.abstract | Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy. This protocol can be applied to any brain tumor-harboring mouse model. For complete details on the use and execution of this protocol, please refer to Alghamri et al. (2021). | |
dc.format.medium | Electronic-eCollection | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation.haspart | 101357 | |
dc.rights | Licence for published version: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Antibody | |
dc.subject | Cancer | |
dc.subject | Cell Biology | |
dc.subject | Flow Cytometry/Mass Cytometry | |
dc.subject | Immunology | |
dc.subject | Model Organisms | |
dc.subject | Neuroscience | |
dc.subject | Single Cell | |
dc.subject | Animals | |
dc.subject | Brain | |
dc.subject | Brain Neoplasms | |
dc.subject | DNA Transposable Elements | |
dc.subject | Disease Models, Animal | |
dc.subject | Glioma | |
dc.subject | Immunophenotyping | |
dc.subject | Mice | |
dc.subject | Tumor Microenvironment | |
dc.title | Murine brain tumor microenvironment immunophenotyping using mass cytometry | |
dc.type | Article | |
dc.identifier.pmid | 35634359 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/174690/2/Murine brain tumor microenvironment immunophenotyping using mass cytometry.pdf | |
dc.identifier.doi | 10.1016/j.xpro.2022.101357 | |
dc.identifier.doi | https://dx.doi.org/10.7302/6421 | |
dc.identifier.source | STAR Protocols | |
dc.description.version | Published version | |
dc.date.updated | 2022-09-15T18:52:50Z | |
dc.identifier.orcid | 0000-0002-8427-4409 | |
dc.identifier.orcid | 0000-0003-2237-2756 | |
dc.identifier.volume | 3 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 101357 | |
dc.identifier.name-orcid | McClellan, BL | |
dc.identifier.name-orcid | Alghamri, MS | |
dc.identifier.name-orcid | Thalla, R | |
dc.identifier.name-orcid | Lowenstein, PR; 0000-0002-8427-4409 | |
dc.identifier.name-orcid | Castro, MG; 0000-0003-2237-2756 | |
dc.working.doi | 10.7302/6421 | en |
dc.owningcollname | Neurosurgery, Department of |
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