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Murine brain tumor microenvironment immunophenotyping using mass cytometry
dc.contributor.authorMcClellan, BL
dc.contributor.authorAlghamri, MS
dc.contributor.authorThalla, R
dc.contributor.authorLowenstein, PR
dc.contributor.authorCastro, MG
dc.coverage.spatialUnited States
dc.date.accessioned2022-09-15T18:53:46Z
dc.date.available2022-09-15T18:53:46Z
dc.date.issued2022-06-17
dc.identifier.issn2666-1667
dc.identifier.issn2666-1667
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pubmed/35634359
dc.identifier.urihttps://hdl.handle.net/2027.42/174690en
dc.description.abstractHere, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy. This protocol can be applied to any brain tumor-harboring mouse model. For complete details on the use and execution of this protocol, please refer to Alghamri et al. (2021).
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherElsevier
dc.relation.haspart101357
dc.rightsLicence for published version: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntibody
dc.subjectCancer
dc.subjectCell Biology
dc.subjectFlow Cytometry/Mass Cytometry
dc.subjectImmunology
dc.subjectModel Organisms
dc.subjectNeuroscience
dc.subjectSingle Cell
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Neoplasms
dc.subjectDNA Transposable Elements
dc.subjectDisease Models, Animal
dc.subjectGlioma
dc.subjectImmunophenotyping
dc.subjectMice
dc.subjectTumor Microenvironment
dc.titleMurine brain tumor microenvironment immunophenotyping using mass cytometry
dc.typeArticle
dc.identifier.pmid35634359
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/174690/2/Murine brain tumor microenvironment immunophenotyping using mass cytometry.pdf
dc.identifier.doi10.1016/j.xpro.2022.101357
dc.identifier.doihttps://dx.doi.org/10.7302/6421
dc.identifier.sourceSTAR Protocols
dc.description.versionPublished version
dc.date.updated2022-09-15T18:52:50Z
dc.identifier.orcid0000-0002-8427-4409
dc.identifier.orcid0000-0003-2237-2756
dc.identifier.volume3
dc.identifier.issue2
dc.identifier.startpage101357
dc.identifier.name-orcidMcClellan, BL
dc.identifier.name-orcidAlghamri, MS
dc.identifier.name-orcidThalla, R
dc.identifier.name-orcidLowenstein, PR; 0000-0002-8427-4409
dc.identifier.name-orcidCastro, MG; 0000-0003-2237-2756
dc.working.doi10.7302/6421en
dc.owningcollnameNeurosurgery, Department of


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Licence for published version: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

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