View Item 

Show simple item record

Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
dc.contributor.authorHuang, X
dc.contributor.authorYang, D
dc.contributor.authorZhang, J
dc.contributor.authorXu, J
dc.contributor.authorChen, YE
dc.coverage.spatialSwitzerland
dc.date.accessioned2022-10-05T14:30:15Z
dc.date.available2022-10-05T14:30:15Z
dc.date.issued2022-07-01
dc.identifier.issn2073-4409
dc.identifier.issn2073-4409
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pubmed/35883629
dc.identifier.urihttps://hdl.handle.net/2027.42/174880en
dc.description.abstractCRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR–Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI
dc.relation.haspartARTN 2186
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCRISPR–Cas9
dc.subjectgene editing
dc.subjecthigh fidelity
dc.subjectoff-target
dc.subjectprotein engineering
dc.subjectspecificity
dc.subjectCRISPR-Associated Protein 9
dc.subjectCRISPR-Cas Systems
dc.subjectEndonucleases
dc.subjectGene Editing
dc.subjectProtein Engineering
dc.titleRecent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
dc.typeArticle
dc.identifier.pmid35883629
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/174880/2/Recent Advances in Improving Gene-Editing Specificity through CRISPR-Cas9 Nuclease Engineering.pdf
dc.identifier.doi10.3390/cells11142186
dc.identifier.doihttps://dx.doi.org/10.7302/6509
dc.identifier.sourceCells
dc.description.versionPublished version
dc.date.updated2022-10-05T14:30:11Z
dc.identifier.orcid0000-0002-1005-848X
dc.identifier.orcid0000-0003-2449-8651
dc.identifier.orcid0000-0001-9452-2878
dc.identifier.orcid0000-0003-2357-7825
dc.identifier.volume11
dc.identifier.issue14
dc.identifier.startpage2186
dc.identifier.name-orcidHuang, X; 0000-0002-1005-848X
dc.identifier.name-orcidYang, D; 0000-0003-2449-8651
dc.identifier.name-orcidZhang, J
dc.identifier.name-orcidXu, J; 0000-0001-9452-2878
dc.identifier.name-orcidChen, YE; 0000-0003-2357-7825
dc.working.doi10.7302/6509en
dc.owningcollnameInternal Medicine, Department of


Files in this item

Name:
Recent Advances in Improving ...
Size:
1.353MB
Format:
PDF
View/Open

Show simple item record

Licence for published version: Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution 4.0 International

Remediation of Harmful Language

The University of Michigan Library aims to describe its collections in a way that respects the people and communities who create, use, and are represented in them. We encourage you to Contact Us anonymously if you encounter harmful or problematic language in catalog records or finding aids. More information about our policies and practices is available at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.