CRISPR/Cas9-mediated TERT disruption in cancer cells
dc.contributor.author | Wen, L | |
dc.contributor.author | Zhao, C | |
dc.contributor.author | Song, J | |
dc.contributor.author | Ma, L | |
dc.contributor.author | Ruan, J | |
dc.contributor.author | Xia, X | |
dc.contributor.author | Chen, YE | |
dc.contributor.author | Zhang, J | |
dc.contributor.author | Ma, PX | |
dc.contributor.author | Xu, J | |
dc.coverage.spatial | Switzerland | |
dc.date.accessioned | 2022-10-05T14:50:44Z | |
dc.date.available | 2022-10-05T14:50:44Z | |
dc.date.issued | 2020-01-02 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/31963842 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/174894 | en |
dc.description.abstract | Mammalian telomere lengths are primarily regulated by telomerase, a ribonucleoprotein consisting of a reverse transcriptase (TERT) and an RNA subunit (TERC). TERC is constitutively expressed in all cells, whereas TERT expression is temporally and spatially regulated, such that in most adult somatic cells, TERT is inactivated and telomerase activity is undetectable. Most tumor cells activate TERT as a mechanism for preventing progressive telomere attrition to achieve proliferative immortality. Therefore, inactivating TERT has been considered to be a promising means of cancer therapy. Here we applied the CRISPR/Cas9 gene editing system to target the TERT gene in cancer cells. We report that disruption of TERT severely compromises cancer cell survival in vitro and in vivo. Haploinsufficiency of TERT in tumor cells is sufficient to result in telomere attrition and growth retardation in vitro. In vivo, TERT haploinsufficient tumor cells failed to form xenograft after transplantation to nude mice. Our work demonstrates that gene editing-mediated TERT knockout is a potential therapeutic option for treating cancer. | |
dc.format.medium | Electronic | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.relation.haspart | ARTN 653 | |
dc.rights | Licence for published version: Creative Commons Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | CRISPR/Cas9 | |
dc.subject | TERT | |
dc.subject | cancer therapy | |
dc.subject | gene editing | |
dc.subject | telomerase | |
dc.subject | Animals | |
dc.subject | CRISPR-Cas Systems | |
dc.subject | Cell Line, Tumor | |
dc.subject | Cell Proliferation | |
dc.subject | Cell Survival | |
dc.subject | Female | |
dc.subject | Gene Knockout Techniques | |
dc.subject | Haploinsufficiency | |
dc.subject | HeLa Cells | |
dc.subject | Humans | |
dc.subject | INDEL Mutation | |
dc.subject | Mice | |
dc.subject | Mice, Nude | |
dc.subject | Neoplasm Transplantation | |
dc.subject | Telomerase | |
dc.subject | Uterine Cervical Neoplasms | |
dc.title | CRISPR/Cas9-mediated TERT disruption in cancer cells | |
dc.type | Article | |
dc.identifier.pmid | 31963842 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/174894/2/CRISPRCas9-Mediated TERT Disruption in Cancer Cells. .pdf | |
dc.identifier.doi | 10.3390/ijms21020653 | |
dc.identifier.doi | https://dx.doi.org/10.7302/6523 | |
dc.identifier.source | International Journal of Molecular Sciences | |
dc.description.version | Published version | |
dc.date.updated | 2022-10-05T14:50:42Z | |
dc.identifier.orcid | 0000-0003-2357-7825 | |
dc.identifier.orcid | 0000-0002-0191-9487 | |
dc.identifier.volume | 21 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | E653 | |
dc.identifier.name-orcid | Wen, L | |
dc.identifier.name-orcid | Zhao, C | |
dc.identifier.name-orcid | Song, J | |
dc.identifier.name-orcid | Ma, L | |
dc.identifier.name-orcid | Ruan, J | |
dc.identifier.name-orcid | Xia, X | |
dc.identifier.name-orcid | Chen, YE; 0000-0003-2357-7825 | |
dc.identifier.name-orcid | Zhang, J | |
dc.identifier.name-orcid | Ma, PX; 0000-0002-0191-9487 | |
dc.identifier.name-orcid | Xu, J | |
dc.working.doi | 10.7302/6523 | en |
dc.owningcollname | Internal Medicine, Department of |
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