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Compromised chondrocyte differentiation capacity in terc knockout mouse embryonic stem cells derived by somatic cell nuclear transfer

dc.contributor.authorChang, WF
dc.contributor.authorWu, YH
dc.contributor.authorXu, J
dc.contributor.authorSung, LY
dc.coverage.spatialSwitzerland
dc.date.accessioned2022-10-05T14:56:13Z
dc.date.available2022-10-05T14:56:13Z
dc.date.issued2019-03-01
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pubmed/30870992
dc.identifier.urihttps://hdl.handle.net/2027.42/174899en
dc.description.abstractMammalian telomere lengths are primarily regulated by telomerase, consisting of a reverse transcriptase protein (TERT) and an RNA subunit (TERC). We previously reported the generation of mouse Terc+/− and Terc−/− embryonic stem cells (ntESCs) by somatic cell nuclear transfer. In the present work, we investigated the germ layer development competence of Terc−/−, Terc+/− and wild-type (Terc+/+) ntESCs. The telomere lengths are longest in wild-type but shortest in Terc−/− ntESCs, and correlate reversely with the population doubling time. Interestingly, while in vitro embryoid body (EB) differentiation assay reveals EB size difference among ntESCs of different genotypes, the more stringent in vivo teratoma assay demonstrates that Terc−/− ntESCs are severely defective in differentiating into the mesodermal lineage cartilage. Consistently, in a directed in vitro chondrocyte differentiation assay, the Terc−/− cells failed in forming Collagen II expressing cells. These findings underscore the significance in maintaining proper telomere lengths in stem cells and their derivatives for regenerative medicine.
dc.format.mediumElectronic
dc.languageeng
dc.publisherMDPI
dc.relation.haspartARTN 1236
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectembryonic stem cells
dc.subjectmesoderm
dc.subjectmouse
dc.subjecttelomerase
dc.subjecttelomeres
dc.subjectAnimals
dc.subjectCartilage
dc.subjectCell Differentiation
dc.subjectCell Nucleus
dc.subjectCells, Cultured
dc.subjectChondrocytes
dc.subjectChondrogenesis
dc.subjectHEK293 Cells
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectMouse Embryonic Stem Cells
dc.subjectNuclear Transfer Techniques
dc.subjectRNA
dc.subjectTelomerase
dc.subjectTelomere
dc.subjectTelomere Homeostasis
dc.titleCompromised chondrocyte differentiation capacity in terc knockout mouse embryonic stem cells derived by somatic cell nuclear transfer
dc.typeArticle
dc.identifier.pmid30870992
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/174899/2/Compromised Chondrocyte Differentiation Capacity in TERC Knockout Mouse Embryonic Stem Cells Derived by Somatic Cell Nuclear.pdf
dc.identifier.doi10.3390/ijms20051236
dc.identifier.doihttps://dx.doi.org/10.7302/6528
dc.identifier.sourceInternational Journal of Molecular Sciences
dc.description.versionPublished version
dc.date.updated2022-10-05T14:56:03Z
dc.identifier.volume20
dc.identifier.issue5
dc.identifier.startpageE1236
dc.identifier.name-orcidChang, WF
dc.identifier.name-orcidWu, YH
dc.identifier.name-orcidXu, J
dc.identifier.name-orcidSung, LY
dc.working.doi10.7302/6528en
dc.owningcollnameInternal Medicine, Department of


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Licence for published version: Creative Commons Attribution 4.0 International
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