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Genetically encoded tools for in vivo G-protein-coupled receptor agonist detection at cellular resolution
dc.contributor.authorKroning, Kayla E.
dc.contributor.authorWang, Wenjing
dc.date.accessioned2022-12-05T16:39:44Z
dc.date.available2024-01-05 11:39:43en
dc.date.available2022-12-05T16:39:44Z
dc.date.issued2022-12
dc.identifier.citationKroning, Kayla E.; Wang, Wenjing (2022). "Genetically encoded tools for in vivo G-protein-coupled receptor agonist detection at cellular resolution." Clinical and Translational Medicine 12(12): n/a-n/a.
dc.identifier.issn2001-1326
dc.identifier.issn2001-1326
dc.identifier.urihttps://hdl.handle.net/2027.42/175200
dc.description.abstractG-protein-coupled receptors (GPCRs) are the most abundant receptor type in the human body and are responsible for regulating many physiological processes, such as sensation, cognition, muscle contraction and metabolism. Further, GPCRs are widely expressed in the brain where their agonists make up a large number of neurotransmitters and neuromodulators. Due to the importance of GPCRs in human physiology, genetically encoded sensors have been engineered to detect GPCR agonists at cellular resolution in vivo. These sensors can be placed into two main categories: those that offer real-time information on the signalling dynamics of GPCR agonists and those that integrate the GPCR agonist signal into a permanent, quantifiable mark that can be used to detect GPCR agonist localisation in a large brain area. In this review, we discuss the various designs of real-time and integration sensors, their advantages and limitations, and some in vivo applications. We also discuss the potential of using real-time and integrator sensors together to identify neuronal circuits affected by endogenous GPCR agonists and perform detailed characterisations of the spatiotemporal dynamics of GPCR agonist release in those circuits. By using these sensors together, the overall knowledge of GPCR-mediated signalling can be expanded.G-protein-coupled receptor (GPCR) agonist sensors utilise downstream GPCR signalling events, such as GPCR conformational changes and protein binding, to give a measurable sensor readout.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherneuromodulation
dc.subject.otherneurotransmission
dc.subject.othergenetically encoded sensor
dc.subject.otherGPCR
dc.titleGenetically encoded tools for in vivo G-protein-coupled receptor agonist detection at cellular resolution
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMedicine (General)
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175200/1/ctm21124_am.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175200/2/ctm21124.pdf
dc.identifier.doi10.1002/ctm2.1124
dc.identifier.sourceClinical and Translational Medicine
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

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