Electroporation-mediated delivery of the FER Gene in the Resolution of Trauma-related Fatal Pneumonia
dc.contributor.author | Vladislav, A Dolgachev | |
dc.contributor.author | Rebecca, Goldberg | |
dc.contributor.author | Suresh, MV | |
dc.contributor.author | Bivin, Thomas | |
dc.contributor.author | Nicholas, Talarico | |
dc.contributor.author | Mark, R Hemmila | |
dc.contributor.author | Krishnan, Raghavendran | |
dc.contributor.author | David, Machado-Aranda | |
dc.coverage.spatial | Jacksonville, Florida | |
dc.date.accessioned | 2023-07-18T18:18:10Z | |
dc.date.available | 2023-07-18T18:18:10Z | |
dc.date.issued | 2016-11-01 | |
dc.identifier.issn | 0969-7128 | |
dc.identifier.issn | 1476-5462 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/27454317 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/177331 | en |
dc.description.abstract | Injured patients with lung contusion (LC) are at risk of developing bacterial pneumonia (PNA) followed by sepsis and death. A recent genome-wide association study (GWAS) showed FER gene expression positively correlating with survival rates among individuals with above conditions. We sought to determine whether electroporation (EP)-mediated delivery of FER gene could indeed improve survival, in a lethal model of combined LC and PNA. C57BL/6 mice sustained unilateral LC, which preceded a 500 Klebsiella colony forming unit (CFU) inoculation by 6 h. In-between these insults, human FER plasmid (pFER) was introduced into the lungs followed by eight EP pulses applied externally (10 ms at 200 V cm-1). Control groups included EP of empty vector (pcDNA3) or Na+/K+-ATPase genes (pPump) and no treatment (LC+PNA). We recorded survival, histology, lung mechanics, bronchial alveolar lavage (BAL) fluid, FER and inflammatory gene expression and bacteriology. The data show that 7-day survival was significantly improved by pFER compared with control groups. pFER increased BAL monocytes and activated antibacterial response genes (nitric oxide synthase (NOS), Fizz). pFER treatment showed decreased lung and blood Klebsiella counts reaching, in some cases, complete sterilization. In conclusion, FER gene delivery promoted survival in LC+PNA mice via recruitment of activated immune cells, improving efficiency of bacterial clearance within contused lung. | |
dc.format.medium | Print-Electronic | |
dc.publisher | Springer Nature | |
dc.subject | Animals | |
dc.subject | Bacterial Load | |
dc.subject | Contusions | |
dc.subject | Electroporation | |
dc.subject | Genetic Therapy | |
dc.subject | Genetic Vectors | |
dc.subject | Humans | |
dc.subject | Klebsiella | |
dc.subject | Male | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Pneumonia, Bacterial | |
dc.subject | Protein-Tyrosine Kinases | |
dc.subject | Sodium-Potassium-Exchanging ATPase | |
dc.title | Electroporation-mediated delivery of the FER Gene in the Resolution of Trauma-related Fatal Pneumonia | |
dc.type | Conference Paper | |
dc.identifier.pmid | 27454317 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/177331/2/Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.pdf | |
dc.identifier.doi | 10.1038/gt.2016.58 | |
dc.identifier.doi | https://dx.doi.org/10.7302/7928 | |
dc.identifier.source | ASC | |
dc.description.version | Published version | |
dc.date.updated | 2023-07-18T18:18:03Z | |
dc.description.filedescription | Description of Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.pdf : Accepted version | |
dc.identifier.volume | 23 | |
dc.identifier.issue | 11 | |
dc.identifier.startpage | 785 | |
dc.identifier.endpage | 796 | |
dc.identifier.name-orcid | Vladislav, A Dolgachev | |
dc.identifier.name-orcid | Rebecca, Goldberg | |
dc.identifier.name-orcid | Suresh, MV | |
dc.identifier.name-orcid | Bivin, Thomas | |
dc.identifier.name-orcid | Nicholas, Talarico | |
dc.identifier.name-orcid | Mark, R Hemmila | |
dc.identifier.name-orcid | Krishnan, Raghavendran | |
dc.identifier.name-orcid | David, Machado-Aranda | |
dc.working.doi | 10.7302/7928 | en |
dc.owningcollname | Surgery, Department of |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.