Epigenetic Regulation of Myofibroblast Differentiation by DNA Methylation.
dc.contributor.author | Hu, B | |
dc.contributor.author | Gharaee-Kermani, M | |
dc.contributor.author | Wu, Z | |
dc.contributor.author | Phan, SH | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2023-10-11T18:16:23Z | |
dc.date.available | 2023-10-11T18:16:23Z | |
dc.date.issued | 2010-01-01 | |
dc.identifier.issn | 0002-9440 | |
dc.identifier.issn | 1525-2191 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/20489138 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/178293 | en |
dc.description.abstract | DNA methylation, a key mechanism of repressing gene expression, is of particular relevance in controlling development and cell differentiation. We analyzed the extent and regulation of DNA methylation of the α-smooth muscle actin (α-SMA) gene to elucidate its potential role in myofibroblast differentiation. These experiments revealed the presence of three CpG islands that were methylated at different levels in fibroblasts, myofibroblasts, and alveolar epithelial type II cells. Coordinately, these cells expressed low, high, or no α-SMA, respectively. In addition, inhibition of DNA methyltransferase activity or knock down of DNA methyltransferase using specific small interfering RNA caused significant induction of α-SMA in fibroblasts. In contrast, induced overexpression of DNA methyltransferase suppressed α-SMA gene expression. Transforming growth factor β induced myofibroblast differentiation was enhanced or suppressed by knockdown or overexpression of DNA methyltransferase, respectively. Finally, in vitro DNA methylation of the α-SMA promoter suppressed its activity. These findings suggest that DNA methylation mediated by DNA methyltransferase is an important mechanism regulating the α-SMA gene expression during myofibroblast differentiation. Copyright © American Society for Investigative Pathology. | |
dc.format.medium | Print-Electronic | |
dc.publisher | Elsevier | |
dc.subject | Actins | |
dc.subject | Animals | |
dc.subject | Cell Differentiation | |
dc.subject | Cells, Cultured | |
dc.subject | DNA Methylation | |
dc.subject | DNA Modification Methylases | |
dc.subject | Epigenesis, Genetic | |
dc.subject | Female | |
dc.subject | Genes, Reporter | |
dc.subject | Muscle, Smooth | |
dc.subject | Myofibroblasts | |
dc.subject | RNA, Small Interfering | |
dc.subject | Rats | |
dc.subject | Rats, Inbred F344 | |
dc.title | Epigenetic Regulation of Myofibroblast Differentiation by DNA Methylation. | |
dc.type | Conference Paper | |
dc.identifier.pmid | 20489138 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/178293/2/Epigenetic regulation of myofibroblast differentiation by DNA methylation.pdf | |
dc.identifier.doi | 10.2353/ajpath.2010.090999 | |
dc.identifier.doi | https://dx.doi.org/10.7302/8682 | |
dc.identifier.source | Am. J. Respir. Crit. Care Med. | |
dc.description.version | Published version | |
dc.date.updated | 2023-10-11T18:16:20Z | |
dc.identifier.orcid | 0000-0002-4691-6490 | |
dc.identifier.orcid | 0000-0002-2177-2427 | |
dc.description.filedescription | Description of Epigenetic regulation of myofibroblast differentiation by DNA methylation.pdf : Published version | |
dc.identifier.volume | 179 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 21 | |
dc.identifier.endpage | 28 | |
dc.identifier.name-orcid | Hu, B; 0000-0002-4691-6490 | |
dc.identifier.name-orcid | Gharaee-Kermani, M; 0000-0002-2177-2427 | |
dc.identifier.name-orcid | Wu, Z | |
dc.identifier.name-orcid | Phan, SH | |
dc.working.doi | 10.7302/8682 | en |
dc.owningcollname | Internal Medicine, Department of |
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