An Artificial Placenta Protects against Lung Injury and Promotes Continued Lung Development in Extremely Premature Lambs
dc.contributor.author | Coughlin, MA | |
dc.contributor.author | Werner, NL | |
dc.contributor.author | Church, JT | |
dc.contributor.author | Perkins, EM | |
dc.contributor.author | Bryner, BS | |
dc.contributor.author | Barks, JD | |
dc.contributor.author | Bentley, JK | |
dc.contributor.author | Hershenson, MB | |
dc.contributor.author | Rabah, R | |
dc.contributor.author | Bartlett, RH | |
dc.contributor.author | Mychaliska, GB | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2024-01-18T19:08:33Z | |
dc.date.available | 2024-01-18T19:08:33Z | |
dc.date.issued | 2019-09-01 | |
dc.identifier.issn | 1058-2916 | |
dc.identifier.issn | 1538-943X | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/30585874 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/192081 | en |
dc.description.abstract | An artificial placenta (AP) utilizing extracorporeal life support (ECLS) could protect premature lungs from injury and promote continued development. Preterm lambs at estimated gestational age (EGA) 114-128 days (term = 145) were delivered by Caesarian section and managed in one of three groups: AP, mechanical ventilation (MV), or tissue control (TC). Artificial placenta lambs (114 days EGA, n = 3; 121 days, n = 5) underwent venovenous (VV)-ECLS with jugular drainage and umbilical vein reinfusion for 7 days, with a fluid-filled, occluded airway. Mechanical ventilation lambs (121 days, n = 5; 128 days, n = 5) underwent conventional MV until failure or maximum 48 hours. Tissue control lambs (114 days, n = 3; 121 days, n = 5; 128 days, n = 5) were sacrificed at delivery. At the conclusion of each experiment, lungs were procured and sectioned. Hematoxylin and eosin (H&E) slides were scored 0-4 in seven injury categories, which were summed for a total injury score. Slides were also immunostained for platelet-derived growth factor receptor (PDGFR)-α and α-actin; lung development was quantified by the area fraction of double-positive tips of secondary alveolar septa. Support duration of AP lambs was 163 ± 9 (mean ± SD) hours, 4 ± 3 for early MV lambs, and 40 ± 6 for late MV lambs. Total injury scores at 121 days were 1.7 ± 2.1 for AP vs. 5.5 ± 1.6 for MV (p = 0.02). Using immunofluorescence, double-positive tip area fraction at 121 days was 0.017 ± 0.011 in AP lungs compared with 0.003 ± 0.003 in MV lungs (p < 0.001) and 0.009 ± 0.005 in TC lungs. At 128 days, double-positive tip area fraction was 0.012 ± 0.007 in AP lungs compared with 0.004 ± 0.004 in MV lungs (p < 0.001) and 0.016 ± 0.009 in TC lungs. The AP is protective against lung injury and promotes lung development compared with mechanical ventilation in premature lambs. | |
dc.format.medium | ||
dc.language | eng | |
dc.publisher | Wolters Kluwer | |
dc.subject | Animals | |
dc.subject | Animals, Newborn | |
dc.subject | Artificial Organs | |
dc.subject | Extracorporeal Membrane Oxygenation | |
dc.subject | Female | |
dc.subject | Lung | |
dc.subject | Lung Injury | |
dc.subject | Placenta | |
dc.subject | Pregnancy | |
dc.subject | Premature Birth | |
dc.subject | Respiration, Artificial | |
dc.subject | Sheep | |
dc.title | An Artificial Placenta Protects against Lung Injury and Promotes Continued Lung Development in Extremely Premature Lambs | |
dc.type | Article | |
dc.identifier.pmid | 30585874 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/192081/2/an_artificial_placenta_protects_against_lung.12.pdf | |
dc.identifier.doi | 10.1097/MAT.0000000000000939 | |
dc.identifier.doi | https://dx.doi.org/10.7302/22081 | |
dc.identifier.source | ASAIO Journal | |
dc.description.version | Published version | |
dc.date.updated | 2024-01-18T19:08:30Z | |
dc.identifier.orcid | 0000-0002-1453-8827 | |
dc.identifier.orcid | 0000-0003-1320-905X | |
dc.identifier.orcid | 0000-0001-8865-7979 | |
dc.identifier.orcid | 0000-0001-9436-5593 | |
dc.identifier.volume | 65 | |
dc.identifier.issue | 7 | |
dc.identifier.startpage | 690 | |
dc.identifier.endpage | 697 | |
dc.identifier.name-orcid | Coughlin, MA | |
dc.identifier.name-orcid | Werner, NL | |
dc.identifier.name-orcid | Church, JT; 0000-0002-1453-8827 | |
dc.identifier.name-orcid | Perkins, EM | |
dc.identifier.name-orcid | Bryner, BS | |
dc.identifier.name-orcid | Barks, JD; 0000-0003-1320-905X | |
dc.identifier.name-orcid | Bentley, JK; 0000-0001-8865-7979 | |
dc.identifier.name-orcid | Hershenson, MB; 0000-0001-9436-5593 | |
dc.identifier.name-orcid | Rabah, R | |
dc.identifier.name-orcid | Bartlett, RH | |
dc.identifier.name-orcid | Mychaliska, GB | |
dc.working.doi | 10.7302/22081 | en |
dc.owningcollname | Surgery, Department of |
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