Long-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope
dc.contributor.author | Zhao, Xintao | |
dc.date.accessioned | 2024-05-22T17:26:14Z | |
dc.date.available | 2024-05-22T17:26:14Z | |
dc.date.issued | 2024 | |
dc.date.submitted | 2024 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/193380 | |
dc.description.abstract | Long-term observation of live cells and activities, including cell dynamics and cellular responses, is a crucial technology in the field of cell studies such as cancer diagnostics and disease therapy. The challenges of long-term imaging fall under four categories: selection of cell type amenable to long-term imaging; precise control of the cellular environment under in-vitro system; imaging system and probe design to overcome photobleaching, phototoxicity, and to achieve high resolution; efficient data extraction and post data analysis process. Here we present a long-term non-bleaching nanoscale imaging method by plasmonic nanoscope. Through plasmonic molecular marker (gold nanorods) labeling and phase intensity separation of nanoprobes, we achieved non-bleaching, nanoscale imaging of biological systems, such as actin networks. While the standard fluorophore-based method limiting the imaging windows to minutes (< 1 hour), using our optimized imaging system, we were able to continuously observe long-term biological dynamics in the window of hours to days as well as achieving sub-10 nm resolution. The innovative nanoscale imaging system utilizing a plasmonic nanoscope has been successfully developed to address challenges encountered in long-term bio-imaging and demonstrated by visualization and quantitative analysis of actin dynamics during the disassembly process by actin binding protein (cofilin). Furthermore, we demonstrated live-cell imaging using nanoscale plasmonic nanoscope which allowed for the exploration of the orientation distribution of extracellular beta-actin network within a cell-division cycle. | |
dc.language.iso | en_US | |
dc.subject | Plasmonics | |
dc.subject | Nanoscope | |
dc.subject | Long-term Bio-imaging | |
dc.subject | Gold nanoparticle | |
dc.subject | Actin | |
dc.subject | SH-SY5Y | |
dc.title | Long-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope | |
dc.type | Thesis | |
dc.description.thesisdegreename | PhD | |
dc.description.thesisdegreediscipline | Electrical and Computer Engineering | |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | |
dc.contributor.committeemember | Lee, Somin Eunice | |
dc.contributor.committeemember | Nagrath, Sunitha | |
dc.contributor.committeemember | Willingale, Louise | |
dc.contributor.committeemember | Yoon, Euisik | |
dc.subject.hlbsecondlevel | Biomedical Engineering | |
dc.subject.hlbsecondlevel | Electrical Engineering | |
dc.subject.hlbtoplevel | Engineering | |
dc.contributor.affiliationumcampus | Ann Arbor | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/193380/1/xintao_1.pdf | |
dc.identifier.doi | https://dx.doi.org/10.7302/23025 | |
dc.identifier.orcid | 0009-0007-5437-2239 | |
dc.identifier.name-orcid | Zhao, Xintao; 0009-0007-5437-2239 | en_US |
dc.working.doi | 10.7302/23025 | en |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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