Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes
dc.contributor.author | Mori, H | |
dc.contributor.author | Peterson, SK | |
dc.contributor.author | Simmermon, RC | |
dc.contributor.author | Overmyer, KA | |
dc.contributor.author | Nishii, A | |
dc.contributor.author | Paulsson, E | |
dc.contributor.author | Li, Z | |
dc.contributor.author | Jen, A | |
dc.contributor.author | Uranga, RM | |
dc.contributor.author | Maung, JN | |
dc.contributor.author | Yacawych, WT | |
dc.contributor.author | Lewis, KT | |
dc.contributor.author | Schill, RL | |
dc.contributor.author | Hetrick, T | |
dc.contributor.author | Seino, R | |
dc.contributor.author | Inoki, K | |
dc.contributor.author | Coon, JJ | |
dc.contributor.author | MacDougald, OA | |
dc.coverage.spatial | Germany | |
dc.date.accessioned | 2024-05-27T12:35:57Z | |
dc.date.available | 2024-05-27T12:35:57Z | |
dc.date.issued | 2024-05-01 | |
dc.identifier.issn | 2212-8778 | |
dc.identifier.issn | 2212-8778 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/38492843 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/193492 | en |
dc.description.abstract | Objective: Exposure of adipocytes to ‘cool’ temperatures often found in the periphery of the body induces expression of Stearoyl-CoA Desaturase-1 (Scd1), an enzyme that converts saturated fatty acids to monounsaturated fatty acids. The goal of this study is to further investigate the roles of Scd in adipocytes. Method: In this study, we employed Scd1 knockout cells and mouse models, along with pharmacological Scd1 inhibition to dissect the enzyme's function in adipocyte physiology. Results: Our study reveals that production of monounsaturated lipids by Scd1 is necessary for fusion of autophagosomes to lysosomes and that with a Scd1-deficiency, autophagosomes accumulate. In addition, Scd1-deficiency impairs lysosomal and autolysosomal acidification resulting in vacuole accumulation and eventual cell death. Blocking autophagosome formation or supplementation with monounsaturated fatty acids maintains vitality of Scd1-deficient adipocytes. Conclusion: This study demonstrates the indispensable role of Scd1 in adipocyte survival, with its inhibition in vivo triggering autophagy-dependent cell death and its depletion in vivo leading to the loss of bone marrow adipocytes. | |
dc.format.medium | Print-Electronic | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation.haspart | 101916 | |
dc.rights | Licence for published version: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | ADCD | |
dc.subject | Autophagy-dependent cell death | |
dc.subject | BMAT | |
dc.subject | BMAds | |
dc.subject | Bone marrow adipocytes | |
dc.subject | Bone marrow adipose tissue | |
dc.subject | MUFA | |
dc.subject | Monounsaturated fatty acid | |
dc.subject | Scd1 | |
dc.subject | Stearoyl CoA Desaturase 1 | |
dc.subject | Stearoyl-CoA Desaturase | |
dc.subject | Animals | |
dc.subject | Mice | |
dc.subject | Autophagy | |
dc.subject | Adipocytes | |
dc.subject | Fatty Acids, Monounsaturated | |
dc.subject | Mice, Knockout | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Lysosomes | |
dc.subject | Cell Survival | |
dc.subject | 3T3-L1 Cells | |
dc.subject | Male | |
dc.subject | Lipid Metabolism | |
dc.subject | Autophagosomes | |
dc.title | Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes | |
dc.type | Article | |
dc.identifier.pmid | 38492843 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/193492/2/Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes.pdf | |
dc.identifier.doi | 10.1016/j.molmet.2024.101916 | |
dc.identifier.doi | https://dx.doi.org/10.7302/23137 | |
dc.identifier.source | Molecular Metabolism | |
dc.description.version | Published version | |
dc.date.updated | 2024-05-27T12:35:51Z | |
dc.identifier.orcid | 0000-0003-0503-3277 | |
dc.identifier.orcid | 0000-0001-6907-7960 | |
dc.description.filedescription | Description of Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes.pdf : Accepted version | |
dc.identifier.volume | 83 | |
dc.identifier.startpage | 101916 | |
dc.identifier.name-orcid | Mori, H; 0000-0003-0503-3277 | |
dc.identifier.name-orcid | Peterson, SK | |
dc.identifier.name-orcid | Simmermon, RC | |
dc.identifier.name-orcid | Overmyer, KA | |
dc.identifier.name-orcid | Nishii, A | |
dc.identifier.name-orcid | Paulsson, E | |
dc.identifier.name-orcid | Li, Z | |
dc.identifier.name-orcid | Jen, A | |
dc.identifier.name-orcid | Uranga, RM | |
dc.identifier.name-orcid | Maung, JN | |
dc.identifier.name-orcid | Yacawych, WT | |
dc.identifier.name-orcid | Lewis, KT | |
dc.identifier.name-orcid | Schill, RL | |
dc.identifier.name-orcid | Hetrick, T | |
dc.identifier.name-orcid | Seino, R | |
dc.identifier.name-orcid | Inoki, K | |
dc.identifier.name-orcid | Coon, JJ | |
dc.identifier.name-orcid | MacDougald, OA; 0000-0001-6907-7960 | |
dc.working.doi | 10.7302/23137 | en |
dc.owningcollname | Molecular and Integrative Physiology, Department of |
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