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Tracking Microcystis N and C Uptake from Urea in Whole Lake Water

dc.contributor.authorVandeVusse, Hadley
dc.contributor.advisorDick, Gregory
dc.date.accessioned2024-05-31T18:13:52Z
dc.date.available2024-05-31T18:13:52Z
dc.date.issued2024-04-25
dc.identifier.urihttps://hdl.handle.net/2027.42/193514
dc.description.abstractLake Erie experiences annual summer cyanobacterial harmful algal blooms (cHABs) dominated by the cyanobacterium, Microcystis. Microcystis species are composed of a wide variety of strains, some of which produce the hepatotoxin microcystin. Lake Erie cHABs result from eutrophication driven primarily by agricultural runoff. About 60% of the world’s nitrogen fertilizer is in the form of urea, which has increased ~100-fold in the last four years. Unlike nitrate and ammonia, which are inorganic sources of nitrogen, urea is an organic source. A previous study found that cultured Microcystis can use both nitrogen and carbon from dual-labeled (15N, 13C) urea. To analyze the potential of carbon and nitrogen uptake from urea in uncultured Western Lake Erie Microcystis, we incubated whole lake water samples with dual-labeled urea. The collection of Microcystis strains sampled from the lake did not incorporate carbon but did incorporate nitrogen. Overall, this work provides further evidence that Microcystis uses urea as a source of nitrogen, but that the use of urea as a carbon source may vary across different Microcystis strains and/or environmental conditions.en_US
dc.language.isoen_USen_US
dc.subjectMicrocystisen_US
dc.subjectLake Erieen_US
dc.subjectcHABsen_US
dc.subjectureaen_US
dc.subjectnitrogenen_US
dc.titleTracking Microcystis N and C Uptake from Urea in Whole Lake Wateren_US
dc.typeThesis
dc.description.thesisdegreenameHonors (Bachelor's)
dc.description.thesisdegreedisciplineEarth and Environmental Sciencesen_US
dc.description.thesisdegreegrantorUniversity of Michiganen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/193514/1/VandeVusse_DeepBlue_Thesis.pdf
dc.identifier.doihttps://dx.doi.org/10.7302/23157
dc.description.filedescriptionDescription of VandeVusse_DeepBlue_Thesis.pdf : Thesis
dc.working.doi10.7302/23157en_US
dc.owningcollnameHonors Theses (Bachelor's)


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