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Novel Inhibitors of E. coli RecA ATPase Activity.
dc.contributor.authorSexton, JZ
dc.contributor.authorWigle, TJ
dc.contributor.authorHe, Q
dc.contributor.authorHughes, MA
dc.contributor.authorSmith, GR
dc.contributor.authorSingleton, SF
dc.contributor.authorWilliams, AL
dc.contributor.authorYeh, LA
dc.coverage.spatialUnited Arab Emirates
dc.date.accessioned2024-10-28T18:58:15Z
dc.date.available2024-10-28T18:58:15Z
dc.date.issued2010-01-26
dc.identifier.issn1875-3973
dc.identifier.issn1875-3973
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pubmed/20648224
dc.identifier.urihttps://hdl.handle.net/2027.42/195413en
dc.description.abstractThe bacterial RecA protein has been implicated as a bacterial drug target not as an antimicrobial target, but as an adjuvant target with the potential to suppress the mechanism by which bacteria gain drug resistance. In order to identify small molecules that inhibit RecA/ssDNA nucleoprotein filament formation, we have adapted the phosphomolybdateblue ATPase assay for high throughput screening to determine RecA ATPase activity against a library of 33,600 compounds, which is a selected representation of diverse structure of 350,000. Four distinct chemotypes were represented among the 40 validated hits. SAR and further chemical synthesis is underway to optimize this set of inhibitors to be used as antimicrobial adjuvant agents. © Sexton et al.
dc.format.mediumElectronic
dc.languageeng
dc.publisherBentham Science Publishers
dc.rightsLicence for published version: Creative Commons Attribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectATPase inhibition
dc.subjectAntibiotic resistance
dc.subjectRecA
dc.subjectSOS response.
dc.subjecthigh throughput screening
dc.subjectmicrobial drug resistance
dc.titleNovel Inhibitors of E. coli RecA ATPase Activity.
dc.typeArticle
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/195413/2/Novel Inhibitors of E. coli RecA ATPase Activity.pdf
dc.identifier.doi10.2174/1875397301004010034
dc.identifier.doihttps://dx.doi.org/10.7302/24608
dc.identifier.sourceCurrent chemical genomics
dc.description.versionPublished version
dc.date.updated2024-10-28T18:58:14Z
dc.identifier.orcid0000-0002-9244-5888
dc.identifier.volume4
dc.identifier.issue1
dc.identifier.startpage34
dc.identifier.endpage42
dc.identifier.name-orcidSexton, JZ; 0000-0002-9244-5888
dc.identifier.name-orcidWigle, TJ
dc.identifier.name-orcidHe, Q
dc.identifier.name-orcidHughes, MA
dc.identifier.name-orcidSmith, GR
dc.identifier.name-orcidSingleton, SF
dc.identifier.name-orcidWilliams, AL
dc.identifier.name-orcidYeh, LA
dc.working.doi10.7302/24608en
dc.owningcollnameInternal Medicine, Department of


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Licence for published version: Creative Commons Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution-NonCommercial 4.0 International

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