Now showing items 51-60 of 1358
Determination of Binding Mode: Intercalation
(Academic PressWiley Periodicals, Inc., 2000-02)
A small molecule can be assumed to bind to DNA by intercalation between base pairs if it causes lengthening and unwinding of the DNA helix and undergoes changes in its spectral properties, such as DNA‐induced hypochromism ...
Ionophore‐Based Biphasic Chemical Sensing in Droplet Microfluidics
(Wiley, 2019-06-11)
Droplet microfluidics is an enabling platform for high‐throughput screens, single‐cell studies, low‐volume chemical diagnostics, and microscale material syntheses. Analytical methods for real‐time and in situ detection of ...
Origin of Chlorophyll Fluorescence in Plants at 55–75°C ¶
(Blackwell Publishing Ltd, 2003-01)
The origin of heat-induced chlorophyll fluorescence rise that appears at about 55–60°C during linear heating of leaves, chloroplasts or thylakoids (especially with a reduced content of grana thylakoids) was studied. This ...
Zwitterionic Surfactant as a Promising Non‐Cytotoxic Carrier for Nanoemulsion‐Based Vaccine Development
(Wiley Periodicals, Inc., 2019-08-23)
Motivated by the lack of noncytotoxic carriers in the current vaccine, we pursued the possibility of using a zwitterionic surfactant as a carrier to improve their delivery efficiency with antigen for a nanoemulsion‐based ...
Early events of Bacillus anthracis germination identified by time-course quantitative proteomics
(WILEY-VCH Verlag, 2006-10)
Germination of Bacillus anthracis spores involves rehydration of the spore interior and rapid degradation of several of the protective layers, including the spore coat. Here, we examine the temporal changes that occur ...
Cellular Delivery of Locked Nucleic Acids (LNAs)
(Wiley Periodicals, Inc., 2002-06)
Locked nucleic acids (LNAs) are RNA derivatives that have an O‐methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high‐affinity binding to complementary sequences. They are synthesized ...
Electron detachment dissociation of fluorescently labeled sialylated oligosaccharides
(WILEY‐VCH Verlag, 2011-12)
We explored the application of electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry to fluorescently labeled sialylated oligosaccharides. Standard sialylated ...
Functionalization of Styrenes by Copper‐Catalyzed Borylation/ ortho‐Cyanation and Silver‐Catalyzed Annulation Processes
(WILEY‐VCH Verlag, 2015-10-19)
An efficient two‐step method for the assembly of indanone derivatives starting from a simple vinyl arene has been developed. The sequence first involves addition of bis(pinacolato)diboron (B2pin2) and N‐cyano‐N‐phenyl‐p‐ ...