Now showing items 21-30 of 53
Production and characterization of antisera raised to the manninotrionate group [[alpha]--Galp-(1-->6)-[alpha]--Galp-(1-->6)--gluconate]
(Elsevier, 1989-06-15)
Manninotrionate [[alpha]--Galp-(1-->6)-[alpha]--Galp-(1-->6)--gluconate] was coupled to bovine serum albumin (BSA), and antisera were raised in rabbits to this carbohydrate--protein conjugate. The antisera were studied ...
New mannose-specific lectins from garlic (Allium sativum) and ramsons (Allium ursinum) bulbs
(Elsevier, 1992-05-22)
Two new mannose-binding lectins were isolated from garlic (Allium sativum, ASA) and ramsons (Allium ursinum, AUA) bulbs, of the family Alliaceae, by affinity chromatography on immobilized mannose. The carbohydrate-binding ...
Effect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cells
(Kluwer Academic Publishers; Springer Science+Business Media, 1988-05)
Hybridoma cells (S3H5/γ2bA2) are found to grow either in suspension or as attached to the surface of cell culture T flask. Cell growth rates and monoclonal antibody (MAB) production rates of both suspended and attached ...
Chemical extraction of indigo from Indigofera tinctoria while attaining biological integrity
(Kluwer Academic Publishers; Springer Science+Business Media, 1999-08)
Indigofera tinctoria was permeabilized with 20% methanol (v/v) at 25 °C and released 8±2 μg indigo g −1 dry plant material (excluding roots). This is equivalent to 42±11% of the total indican within the cells. The plants ...
Observations consistent with autocrine stimulation of hybridoma cell growth and implications for large-scale antibody production
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-04)
Existence of autocrine growth factors (aGFs) may influence the serum requirement for growth of hybridoma cells and thus significantly influence process economics. For the murine hybridoma cell line S3H5/γ2bA2, critical ...
Synthesis of p-nitrophenyl 2-O-[alpha]--mannopyranosyl-[alpha]--mannopyranoside and p-nitrophenyl 6-O-[alpha]--mannopyranosyl-[alpha]--mannopyranoside
(Elsevier, 1979-12)
Crystalline 2-O-[alpha]--mannopyranosyl-[beta]--mannopyranose octaacetate was synthesized by condensation of tetra-O-acetyl-[alpha]--mannopyranosyl bromide (3) with 1,3,4,6-tetra-O-acetyl-[beta]--mannopyranose. ...
Gas-liquid chromatography of carbohydrate derivatives: the separation and quantitative determination of synthetic and naturally occurring glycosyl derivatives of amino acids
(Elsevier, 1972-05)
A gas-liquid chromatographic separation of the trimethylsilyl derivatives of several synthetic and naturally occurring glycosyl derivatives of amino acids is described. This technique may be used for the qualitative and ...
Preparation of cyrstalline [alpha]-kojibiose octaacetate from dextran B-1299-S: Its conversion into p-nitrophenyl and p-isothiocyanatophenyl [beta]-kojibioside
(Elsevier, 1973-03)
Acetolysis of dextran B-1299-S provides a preparative approach to crystalline [alpha]-kojibiose octaacetate. The latter was transformed via crystalline hepta-O-acetyl-[alpha]-kojibiosyl bromide into p-nitrophenyl and ...