Regulation of ovarian steroidogenesis : The disparity between 125I-labelled choriogonadotropin binding, cyclic adenosine 3',5'-monophosphate formation and progesterone synthesis in the rat ovary
dc.contributor.author | Clark, M. R. | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2006-04-07T16:26:29Z | |
dc.date.available | 2006-04-07T16:26:29Z | |
dc.date.issued | 1976-08-24 | en_US |
dc.identifier.citation | Clark, M. R., Menon, K. M. J. (1976/08/24)."Regulation of ovarian steroidogenesis : The disparity between 125I-labelled choriogonadotropin binding, cyclic adenosine 3',5'-monophosphate formation and progesterone synthesis in the rat ovary." Biochimica et Biophysica Acta (BBA) - General Subjects 444(1): 23-32. <http://hdl.handle.net/2027.42/21696> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1W-47N6T05-97/2/87fa4a771a802856fe952fc756e9ac76 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21696 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=182265&dopt=citation | en_US |
dc.description.abstract | The binding of 125I-labeled human choriogonadotropin, formation of cyclic adenosine 3',5'-monophosphate (cyclic AMP), and synthesis of progesterone were examined in ovarian cells from immature rats. Collagenase dispersed ovarian cells were found to respond specifically to lutropin-like activity. The equilibrium dissociation constant (Kd) for the binding of 125I-labelled choriogonadotropin was 1.7 [middle dot] 101-10 M. Progesterone synthesis was increased at least 40-fold and cyclic AMP formation 10-fold in response to maximum hormonal stimulation. The concentration of choriogonadotropin which stimulated progesterone synthesis maximally in Eagle's minimum essential medium -0.1% gelatin (2 ng/ml), resulted in minimal (less than 30% of maximum) increases in cyclic AMP accumulation and hormone bindind. Similarly, binding of choriogonadotropin was not saturated at a hormone concentration (50 ng/ml) that stimulated maximal cyclic AMP formation. These results are consistent with the existence of receptor reserve in the ovarian cell. A marked shift in the dose vs. response relationship for progesterone synthesis occurred when fetal calf serum was used to supplemen Eagle's minimum essential medium, however. Under these experimental conditions, progesterone synthesis reached a maximum at a hormone concentration of the same order of magnitude as did cyclic AMP formation. It is concluded that the degree of spare receptor effect observed may depend not only on an absolute amount of excess receptor, but also on the readiness of the system to respond in a given fashion. | en_US |
dc.format.extent | 599744 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Regulation of ovarian steroidogenesis : The disparity between 125I-labelled choriogonadotropin binding, cyclic adenosine 3',5'-monophosphate formation and progesterone synthesis in the rat ovary | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.identifier.pmid | 182265 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21696/1/0000087.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-4165(76)90220-8 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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