Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis
dc.contributor.author | De Vries, F. A. J. | en_US |
dc.contributor.author | Collins, Carolyn J. | en_US |
dc.contributor.author | Jackson, David A. | en_US |
dc.date.accessioned | 2006-04-07T16:27:27Z | |
dc.date.available | 2006-04-07T16:27:27Z | |
dc.date.issued | 1976-07-02 | en_US |
dc.identifier.citation | De Vries, F. A. J., Collins, Carolyn J., Jackson, David A. (1976/07/02)."Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis." Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 435(3): 213-227. <http://hdl.handle.net/2027.42/21728> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73G8-47TG50F-131/2/b4df2e4bf4fd0f0ab416a6bef383f51c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21728 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=181070&dopt=citation | en_US |
dc.description.abstract | DNA molecules cut with endonuclease R Eco RI can be joined at Eco RI cleavage sites by incubation with polynucleotide ligase. In order to define the optimum conditions for this reaction, linear Simian Virus 40 DNA molecules (SV40(LRI)) produced by endonuclease R Eco RI cleavage of SV40 form I DNA were joined using polynucleotide ligases specified by bacteriophage T4 and Escherichia coli. We have determined that the concentration of the substrate DNA molecules is the most important factor determining the distribution of covalently joined product molecules into a variety of circular and linear monomeric and oligomeric species. | en_US |
dc.format.extent | 940263 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology, University of Michigan Medical School, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Microbiology, University of Michigan Medical School, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Microbiology, University of Michigan Medical School, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.identifier.pmid | 181070 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21728/1/0000120.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2787(76)90103-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.