Changes in structural integrity of heart DNA from aging mice
dc.contributor.author | Chetsanga, Christopher J. | en_US |
dc.contributor.author | Tuttle, Melissa | en_US |
dc.contributor.author | Jacoboni, Ann | en_US |
dc.date.accessioned | 2006-04-07T16:28:04Z | |
dc.date.available | 2006-04-07T16:28:04Z | |
dc.date.issued | 1976-06-15 | en_US |
dc.identifier.citation | Chetsanga, Christopher J., Tuttle, Melissa, Jacoboni, Ann (1976/06/15)."Changes in structural integrity of heart DNA from aging mice." Life Sciences 18(12): 1405-1412. <http://hdl.handle.net/2027.42/21749> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T99-475TF8M-5P/2/819f3ad83dc7b9767691baaf859a6169 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21749 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=940421&dopt=citation | en_US |
dc.description.abstract | The state of the structural integrity of the DNA from mouse myocardial cells has been investigated by utilizing both CsCl density gradient sedimentation and digestion by S1 endonuclease from . The DNA from myocardial cells of young mice sedimented in a narrow peak at the expected density of 1.701 g/cm3, while the DNA from the heart cells of senescent mice became broadly distributed in CsCl gradients, banding even more multimodally in alkaline sucrose gradients. This mode of sedimentation indicates that old mouse DNA becomes partially fragmented. When the native DNA of myocardial cells from 6, 20 and 30 month old mice was treated with single-strand specific S1 endonuclease, it was the DNA from the senescent mice that showed a progressive increase in sensitivity to digestion by the enzyme. The results indicate that the heart DNA of aging mice develops single-stranded gaps in addition to a breakdown into differently sized fragments. | en_US |
dc.format.extent | 394816 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Changes in structural integrity of heart DNA from aging mice | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Biology, Department of Natural Sciences University of Michigan-Dearborn, Dearborn, Michigan 48128, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Biology, Department of Natural Sciences University of Michigan-Dearborn, Dearborn, Michigan 48128, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Biology, Department of Natural Sciences University of Michigan-Dearborn, Dearborn, Michigan 48128, USA | en_US |
dc.identifier.pmid | 940421 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21749/1/0000143.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0024-3205(76)90357-X | en_US |
dc.identifier.source | Life Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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