Spectral intermediates in the reaction of oxygen with purified liver microsomal cytochrome P-450
dc.contributor.author | Peter Guengerich, F. | en_US |
dc.contributor.author | Ballou, David P. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-07T16:28:11Z | |
dc.date.available | 2006-04-07T16:28:11Z | |
dc.date.issued | 1976-06-07 | en_US |
dc.identifier.citation | Peter Guengerich, F., Ballou, David P., Coon, Minor J. (1976/06/07)."Spectral intermediates in the reaction of oxygen with purified liver microsomal cytochrome P-450." Biochemical and Biophysical Research Communications 70(3): 951-956. <http://hdl.handle.net/2027.42/21753> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DMXBND-1DP/2/79bc4b98fdc2e1faca63d92b57e96e72 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21753 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=938535&dopt=citation | en_US |
dc.description.abstract | Stopped flow spectrophotometry has shown the occurrence of two distinct spectral intermediates in the reaction of oxygen with the reduced form of highly purified cytochrome P-450 from liver microsomes. As indicated by difference spectra, Complex I (with maxima at 430 and 450 nm) is rapidly formed and then decays to form Complex II (with a broad maximum at 440 nm), which resembles the intermediate seen in steady state experiments. In the reaction sequence, P-450LMredComplex I-->Complex II-->P-450LMox the last step is rate-limiting. The rate of that step is inadequate to account for the known turnover number of the enzyme in benzphetamine hydroxylation unless NADPH-cytochrome P-450 reductase or cytochrome is added. The latter protein does not appear to function as an electron carrier in this process. | en_US |
dc.format.extent | 389904 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Spectral intermediates in the reaction of oxygen with purified liver microsomal cytochrome P-450 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 938535 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21753/1/0000147.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(76)90684-7 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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