View Item 

Show simple item record

A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels
dc.contributor.authorEckhardt, Allen E.en_US
dc.contributor.authorHayes, Colleen E.en_US
dc.contributor.authorGoldstein, Irwin J.en_US
dc.date.accessioned2006-04-07T16:28:41Z
dc.date.available2006-04-07T16:28:41Z
dc.date.issued1976-05-21en_US
dc.identifier.citationEckhardt, Allen E., Hayes, Colleen E., Goldstein, Irwin J. (1976/05/21)."A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels." Analytical Biochemistry 73(1): 192-197. <http://hdl.handle.net/2027.42/21770>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DVNNVN-S5/2/4b3baee3433787824d631ede7f0f8062en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/21770
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=942095&dopt=citationen_US
dc.description.abstractA sensitive method is described for the detection of glycoproteins after polyacrylamide gel electrophoresis. Dansyl hydrazine is condensed with aldehydic functions generated by periodate oxidation of protein bound carbohydrate. The resulting hydrazones are reduced to the stable hydrazine derivatives with sodium borohydride. After destaining, the fluorescent-labeled glycoproteins are visualized with a long-range uv light source. The dansyl hydrazine staining technique was shown to detect less than 40 ng of carbohydrate bound to Bandeiraea simplicifolia lectin.en_US
dc.format.extent1724890 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gelsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid942095en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/21770/1/0000164.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(76)90154-8en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000164.pdf
Size:
1.644MB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.