Sedimentation behavior of solubilized gonadotropin receptor from plasma membranes of bovine corpus luteum
dc.contributor.author | Thambyrajah, V. | en_US |
dc.contributor.author | Azhar, Salman | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2006-04-07T16:29:50Z | |
dc.date.available | 2006-04-07T16:29:50Z | |
dc.date.issued | 1976-03-25 | en_US |
dc.identifier.citation | Thambyrajah, V., Azhar, Salman, Menon, K. M. J. (1976/03/25)."Sedimentation behavior of solubilized gonadotropin receptor from plasma membranes of bovine corpus luteum." Biochimica et Biophysica Acta (BBA) - General Subjects 428(1): 35-44. <http://hdl.handle.net/2027.42/21805> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1W-47MCSTF-VX/2/2e6562f9f5e1ae94000a2ecf5177c622 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21805 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=177061&dopt=citation | en_US |
dc.description.abstract | The gonadotropin receptors associated with plasma membrane fractions were solubilized by detergents, including Triton X-100, Lubrol WX, Lubrol PX and sodium deoxycholate before and after equilibration with 125I-labelled human chorionic gonadotropin. The binding activity remained in solution even after centrifugation at 300 000 x g for 3 h. The solubilized gonadotropin receptor or gonadotropin receptor complex was characterized by gel filtration and sucrose density gradient centrifugation. Sucrose density gradient centrifugation of solubilized gonadotropin-receptor complex in the presence of Triton X-100 had a sedimentation coefficient of 6.5 S whereas the solubilized uncomplexed receptor had a sedimentation coefficient of 5.1 S. In the absence of the detergent, solubilized hormone receptor complex from plasma membrane fractions I and II sedimented with a apparent sedimentation coefficient of 6.6 S and 7.4 S, respectively. Similary, the free receptor also showed higher sedimentation profile with a apparent sedimentation coefficient of 6.7 S for fraction I and 7.2 S for fraction II. Treatment of plasma membranes with phospholipase A and C inhibited the binding of 125I-labelled human chorionic gonadotropin in a dose dependent manner, whereas phospholipase D was without any effect. Doses of 1.4 mI.U. of phospholipase A or 0.6 mI.U. of phospholipase C were required to produce 50% inhibition of the binding activity. These phospholipases had no effect on the performed 125I-labelled human chorionic gonadotropin-receptor complex nor on the sedimentation profile of solubilized gonadotropin receptor complex. | en_US |
dc.format.extent | 561890 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Sedimentation behavior of solubilized gonadotropin receptor from plasma membranes of bovine corpus luteum | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.identifier.pmid | 177061 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21805/1/0000205.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-4165(76)90106-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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