Cooperative effects of bacterial mutations affecting [lambda] N gene expression : II. Isolation and characterization of mutations in the rif region
dc.contributor.author | Baumann, Marlene F. | en_US |
dc.contributor.author | Friedman, David I. | en_US |
dc.date.accessioned | 2006-04-07T16:32:05Z | |
dc.date.available | 2006-04-07T16:32:05Z | |
dc.date.issued | 1976-08 | en_US |
dc.identifier.citation | Baumann, Marlene F., Friedman, David I. (1976/08)."Cooperative effects of bacterial mutations affecting [lambda] N gene expression : II. Isolation and characterization of mutations in the rif region." Virology 73(1): 128-138. <http://hdl.handle.net/2027.42/21880> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4CMY801-2M/2/edb76f52af2948fb93810c62ad5241b0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21880 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=785803&dopt=citation | en_US |
dc.description.abstract | We report the isolation and initial characterization of a class of mutations, Snu, that map near the rif locus on the Escherichia coli chromosome. Snu mutations inhibit the growth of phage [lambda], an effect primarily seen when Snu mutations are combined with another class of mutations, nus. Nus mutations have previously been shown to inhibit the expression of the N gene product of [lambda], and the experiments reported here suggest that Snu mutations add to this inhibitory effect.One Snu mutation, Snu-9, was shown to cause bacterial growth to be temperature-sensitive. This suggests that, at least, some Snu mutations may be in genes coding for a function essential for bacterial growth. Since genes coding for the [beta] and [beta]' subunits of RNA polymerase map in this region, we recognize that Snu mutations might alter either of these subunits of RNA polymerase. Complementation studies demonstrate that Snu+ is dominant to Snu-, indicating that the mutant phenotype is due to the partial loss of a function necessary for full N expression.Although the hosts carrying Snu and nus mutations (called Supernus) severely restrict the growth of phage which express the N function of [lambda], they do not show any increased inhibitory effect on the growth of [lambda]immP22 and [lambda]imm21, phages which express N functions different from that of [lambda]. However, Supernus hosts do restrict the growth of a [lambda] variant that can grow well in bacteria carrying either component mutation, Snu- or nus-. The restrictive effect of the Supernus strain is far greater than would be expected if the restriction was due to an additive effect of the two component mutations. This implies that there might be an interaction between Snu and nus products and that the Supernus phenotype results from an interference with this interaction. | en_US |
dc.format.extent | 987289 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cooperative effects of bacterial mutations affecting [lambda] N gene expression : II. Isolation and characterization of mutations in the rif region | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology, Medical School, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology, Medical School, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 785803 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21880/1/0000286.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(76)90067-2 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.