Distribution of bromouracil in the pyrimidine oligonucleotides of drosophila DNA
dc.contributor.author | Rizki, Rose M. | en_US |
dc.contributor.author | Rizki, Tahir M. | en_US |
dc.date.accessioned | 2006-04-07T16:33:40Z | |
dc.date.available | 2006-04-07T16:33:40Z | |
dc.date.issued | 1975-12 | en_US |
dc.identifier.citation | Rizki, Rose M., Rizki, T. M. (1975/12)."Distribution of bromouracil in the pyrimidine oligonucleotides of drosophila DNA." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 30(3): 343-353. <http://hdl.handle.net/2027.42/21933> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T2C-47MKJ5Y-4X/2/652d6a1469778e2e3e56839b779bfb7e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21933 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=811984&dopt=citation | en_US |
dc.description.abstract | 1. DNA was isolated from Drosophilia melanogaster larvae fed radioactvie thymidine (TdR), bromodeoxyuridine (BUdR), or one of these nucleosides together with 5-fluorouracil (FU) under identical conditions. The four DNA samples designated T-DNA, B-DNA, T(F)-DNA, and B(F)-DNA respectively were hydrolyzed with formic acid-diphenylamine, and the pyrimidine oligonucleotides of various chain lenghts (isostichs) were fractionated by DEAE-cellulose chromatography. The distributions of the labeled thymine and bromouracil (BU) residues among the pyrimidine isostichs were determined, and compared for the four DNAs. These comparisons indicated the following:2. () Similarity of T-DNA and T(F)-DNA with respect to labeled thymine distribution among the pyrimidine isostichs (using the mononucleotide to pentanucleotide tracts for statistical comparisons) showed that TdR entering DNA synthesis via the salvage pathway has the same distribution whether the synthetic pathway is operating normally or is inhibited by FU treatment.3. (2) Quantitative comparison of the distribution of BU among the isostichs of B-DNA and B(F)-DNA with the distribution of labeled thymine in T-DNA showed significant differences indicating that the organism does not accept BUdR indiscriminately at all thymine sites for DNA synthesis.4. (3) Comparison of the distribution of BU in B-DNA and B(F)-DNA showed that isostichs and 3 differ significantly. The elution profiles of the isostichs also differed qualitatively between these DNAs. This analysis confirms differential incorporation of BUdR into Drosophila DNA in the presence of a thymidylate inhibitor.5. The observations on BUdR incorporation in Drosophila DNA have been discussed with relation to the high frequency of somatic cell mutations induced in imaginal disc cells by treatment with the analog in the presence of inhibitors of thymidylate synthetase. | en_US |
dc.format.extent | 682220 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Distribution of bromouracil in the pyrimidine oligonucleotides of drosophila DNA | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Zoology, The University of Michigan, Ann Arbor, Michigan 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Zoology, The University of Michigan, Ann Arbor, Michigan 48104, U.S.A. | en_US |
dc.identifier.pmid | 811984 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21933/1/0000340.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0027-5107(75)90005-6 | en_US |
dc.identifier.source | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.