Properties of a partially-purified preparation of the prostaglandin-forming oxygenase from sheep vesicular gland
dc.contributor.author | Rome, Leonard H. | en_US |
dc.contributor.author | Lands, William E. M. | en_US |
dc.date.accessioned | 2006-04-07T16:34:43Z | |
dc.date.available | 2006-04-07T16:34:43Z | |
dc.date.issued | 1975-11 | en_US |
dc.identifier.citation | Rome, Leonard H., Lands, William E. M. (1975/11)."Properties of a partially-purified preparation of the prostaglandin-forming oxygenase from sheep vesicular gland." Prostaglandins 10(5): 813-824. <http://hdl.handle.net/2027.42/21964> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T3H-47MJJG3-68/2/74ece9156d6d67d4df66082fcf4cbc10 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21964 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=814584&dopt=citation | en_US |
dc.description.abstract | The fatty acid oxygenase of sheep vesicular glands was solubilized with Tween-40 and purified 60-fold using ammonium sulfate precipitation and DEAE-cellulose chromatography. Glycerol (50%) stabilized the activity at all stages of purification and allowed long-term storage at -60[deg]. The partially purified enzyme contained less than 0.7 nmoles of iron per mg of protein and less than 0.1 nmole of copper per mg of protein. Although the KI values for aspirin, BL-2338, flurbiprofen and ibuprofen remained relatively unchanged during purification, the apparent KI value for inhibition by indomethacin decreased from 120 to 2.7 [mu]. | en_US |
dc.format.extent | 487291 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Properties of a partially-purified preparation of the prostaglandin-forming oxygenase from sheep vesicular gland | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 814584 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21964/1/0000373.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0090-6980(75)90010-6 | en_US |
dc.identifier.source | Prostaglandins | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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