View Item 

Show simple item record

Preparation of 32P-labeled inositides and their degradation by soluble kidney enzymes
dc.contributor.authorLapetina, Eduardo G.en_US
dc.contributor.authorSeguin, Edward B.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2006-04-07T16:36:28Z
dc.date.available2006-04-07T16:36:28Z
dc.date.issued1975-07-22en_US
dc.identifier.citationLapetina, Eduardo G., Seguin, Edward B., Agranoff, Bernard W. (1975/07/22)."Preparation of 32P-labeled inositides and their degradation by soluble kidney enzymes." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 398(1): 118-124. <http://hdl.handle.net/2027.42/22022>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T1X-47F70NW-43/2/4ff14498288285a62471ebf472ea45d0en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/22022
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=238640&dopt=citationen_US
dc.description.abstractA method is described for the preparation of radioactive inositol lipids for studies of their enzymic degradation. Kidney cytosol fractions have been used to produce diesteratic cleavage. High voltage electrophoresis at pH 4.3 is used to separate -myoinositol 1: 2-cyclic phosphate and -myoinositol 1-phosphate from hydrolysis of phosphatidylinositol. Radioactivity co-migrating with myoinositol diphosphate and triphosphate is separated by electrophoresis at pH 1.5 following enzymatic hydrolysis of phosphatidylinositol phosphate and phosphatidylinositol diphosphate. Relative activities for hydrolysis of the various inositides suggest the presence of more than one phosphodiesterase.en_US
dc.format.extent671576 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePreparation of 32P-labeled inositides and their degradation by soluble kidney enzymesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Mental Health Research Institute, The University of Michigan, Neuroscience Laboratory Building, Ann Arbor, Mich. 48104, U.S.Aen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Mental Health Research Institute, The University of Michigan, Neuroscience Laboratory Building, Ann Arbor, Mich. 48104, U.S.Aen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Mental Health Research Institute, The University of Michigan, Neuroscience Laboratory Building, Ann Arbor, Mich. 48104, U.S.Aen_US
dc.identifier.pmid238640en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/22022/1/0000438.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0005-2760(75)90175-7en_US
dc.identifier.sourceBiochimica et Biophysica Actaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000438.pdf
Size:
655.8KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.