Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic enzyme activities
dc.contributor.author | Hansen, Eric J. | en_US |
dc.contributor.author | Juni, Elliot | en_US |
dc.date.accessioned | 2006-04-07T16:36:31Z | |
dc.date.available | 2006-04-07T16:36:31Z | |
dc.date.issued | 1975-07-22 | en_US |
dc.identifier.citation | Hansen, Eric J., Juni, Elliot (1975/07/22)."Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic enzyme activities." Biochemical and Biophysical Research Communications 65(2): 559-566. <http://hdl.handle.net/2027.42/22024> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G3D5KD-77/2/3cbfe32c7c1dc129ba7d77582e9fb966 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22024 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=238534&dopt=citation | en_US |
dc.description.abstract | SummaryWhen Escherichia coli utilizes C4-dicarboxylic acids as sole sources of carbon and energy for growth, phosphoenolpyruvate is synthesized by means of phosphoenolpyruvate carboxykinase or by sequential action of malic enzyme and phosphoenolpyruvate synthetase. Chemical mutagenesis of a strain of E. coli K-12 lacking phosphoenolpyruvate carboxykinase activity yielded a mutant which grows very slowly on malate-mineral medium and lacks NAD-linked malic enzyme activity as well as phosphoenolpyruvate carboxykinase activity. A revertant of this double mutant which possesses elevated levels of NADP-linked malic enzyme activity grows well on malate-mineral medium. Further mutagenesis of this revertant produced a mutant which grows very slowly on malate-mineral medium and lacks NADP-linked malic enzyme activity as well as phosphoenolpyruvate carboxykinase and NAD-linked malic enzyme activities. | en_US |
dc.format.extent | 372149 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic enzyme activities | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology The University of Michigan Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology The University of Michigan Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 238534 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22024/1/0000440.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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