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Use of [1 or 3-3H, U-14c]glucose to estimate the synthesis of glycerolipids via acyl dihydroxyacetone phosphate

dc.contributor.authorPollock, Robert J.en_US
dc.contributor.authorHajra, Amiya K.en_US
dc.contributor.authorFolk, William R.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2006-04-07T16:36:33Z
dc.date.available2006-04-07T16:36:33Z
dc.date.issued1975-07-22en_US
dc.identifier.citationPollock, Robert J., Hajra, Amiya K., Folk, William R., Agranoff, Bernard W. (1975/07/22)."Use of [1 or 3-3H, U-14c]glucose to estimate the synthesis of glycerolipids via acyl dihydroxyacetone phosphate." Biochemical and Biophysical Research Communications 65(2): 658-664. <http://hdl.handle.net/2027.42/22025>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4G3D5KD-7R/2/0e0706a5ffd81fa9d6fe736d18c52459en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/22025
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1170867&dopt=citationen_US
dc.description.abstractSummaryBHK-21-cl3 fibroblasts were incubated with [1-3H, U-14c]glucose or [3-3H, U-14c]glucose to produce intracellular [3H]NADPH via the phosphogluconate pathway. 3H and 14C were then determined at the three positions of glycerol in glycerol phosphate, saponifiable glycerolipids, alkyl ether glycerolipids and plasmalogens. The 3H/14C ratio at C-2 in glycerol of saponifiable glycerolipids is 2-10 fold greater than in glycerol phosphate and approaches the ratio found in ether-containing glycerolipids. This suggests that a significant fraction of the glycerolipids in BHK-21-cl3 fibroblasts is synthesized via acyl dihydroxyacetone phosphate.en_US
dc.format.extent335705 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleUse of [1 or 3-3H, U-14c]glucose to estimate the synthesis of glycerolipids via acyl dihydroxyacetone phosphateen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.identifier.pmid1170867en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/22025/1/0000441.pdfen_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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