Properties of the genome in normal and SV-40 transformed WI-38 human diploid fibroblasts. III. Turnover of nonhistone chromosomal proteins and their phosphate groups
dc.contributor.author | Krause, Margarida O. | en_US |
dc.contributor.author | Kleinsmith, Lewis J. | en_US |
dc.contributor.author | Stein, Gary S. | en_US |
dc.date.accessioned | 2006-04-07T16:38:40Z | |
dc.date.available | 2006-04-07T16:38:40Z | |
dc.date.issued | 1975-04-01 | en_US |
dc.identifier.citation | Krause, Margarida O., Kleinsmith, Lewis J., Stein, Gary S. (1975/04/01)."Properties of the genome in normal and SV-40 transformed WI-38 human diploid fibroblasts. III. Turnover of nonhistone chromosomal proteins and their phosphate groups." Life Sciences 16(7): 1047-1058. <http://hdl.handle.net/2027.42/22096> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T99-479DNRB-F7/2/60ec9cbf929a77c2d2057b9d398f7e00 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22096 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=166261&dopt=citation | en_US |
dc.description.abstract | The turnover of nonhistone chromosomal proteins and their phosphate groups was compared in normal and in SV-40 virus transformed WI-38 human diploid fibroblasts. Cells were pulse labelled with tryptophan-3H and 32P for 30 minutes and the specific activities of tryptophan-3H and 32P in the various molecular weight classes of nonhistone chromosomal proteins were determined during the first four hours following termination of labelling. While a rapid turnover of high molecular weight nonhistone polypeptides (142, 000 to 200, 000 Daltons) is evident after one hour in SV_40 transformed cells, the specific activities of these nonhistone chromosomal polypeptides are not significantly decreased in normal cells. In contrast, a rapid turnover of low molecular weight (30, 000 to 51, 000 Daltons) nonhistone chromosomal proteins occurs during the first hour in normal WI-38 cells with no corresponding decrease in the specific activities of these proteins in SV-40 transformed cells. There is no apparent net turnover of phosphate groups on nonhistone chromosomal proteins in either normal or SV-40 transformed cells four hours following pulse labelling. Rather, during the first four hours significant fluctuations are observed in the 32P specific activities of defined molecular weight fractions. Taken together with previous reports of differences in the composition, synthesis and phosphorylation of nonhistone chromosomal proteins in normal and SV-40 transformed human diploid cells the present results further indicate the complex nature of the alterations in these proteins which accompany viral transformation. | en_US |
dc.format.extent | 455515 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Properties of the genome in normal and SV-40 transformed WI-38 human diploid fibroblasts. III. Turnover of nonhistone chromosomal proteins and their phosphate groups | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Zoology, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Florida, Gainsville, Florida 32610, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Florida, Gainsville, Florida 32610, USA | en_US |
dc.identifier.pmid | 166261 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22096/1/0000520.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0024-3205(75)90189-7 | en_US |
dc.identifier.source | Life Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.