Stimulatory effect of gonadotropins on the synthesis of adenosine 3' :5'-cyclic monophosphate and progesterone by suspensions of rat ovarian interstitial cells
dc.contributor.author | Kawano, Alberto | en_US |
dc.contributor.author | Gunaga, K. P. | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2006-04-07T16:38:51Z | |
dc.date.available | 2006-04-07T16:38:51Z | |
dc.date.issued | 1975-03-14 | en_US |
dc.identifier.citation | Kawano, Alberto, Gunaga, K. P., Menon, K. M. J. (1975/03/14)."Stimulatory effect of gonadotropins on the synthesis of adenosine 3' :5'-cyclic monophosphate and progesterone by suspensions of rat ovarian interstitial cells." Biochimica et Biophysica Acta (BBA) - General Subjects 385(1): 88-100. <http://hdl.handle.net/2027.42/22102> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1W-47MNXGD-1DX/2/65d7e93d9a8d1465e5a433bf85f1593f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22102 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=164926&dopt=citation | en_US |
dc.description.abstract | A cell suspension was prepared from immature rat ovaries by treatment with trypsin and collagenase. The isolated cells were capable of converting [8-14C]adenine to cyclic [14C]AMP and the rate of this conversion was stimulated in vitro by luteinizing hormone and human chorionic gonadotropine, but not by prolactin, norepinephrine, dopamine or albumin. The accumulation of progesterone was also measured in these cells by radioimmunoassay. In vitro addition of luteinizing hormone stimulated the accumulation of radioimmunoassayable progesterone. The conversion of [8-14C]adenine to cyclic [14C]AMP showed a rapid increase during the first 30 min of the incubation period when luteinizing hormone was added to the incubation medium. Progesterone accumulation in response to the same dose of luteinizing hormone showed a lag period for the first 30 min of incubation after which there was an increase up to 2 h. The luteinizing hormone-induced progesterone accumulation was sensitive to puromycin, but there was no effect on the luteinizing hormone-induced increase in cyclic [14C]AMP formation from [8-14C]-adenine. Actinomycin D also inhibited the luteinizing hormone-induced progesterone accumulation, with no effect on cyclic AMP formation. The results suggest that the luteinizing hormone-induced progesterone accumulation in rat ovarian interstitial cell suspension is preceded by an increased accumulation of cyclic AMP and that the accumulation of steroid under the influence of luteinizing hormone involve processes sensitive to puromycin and antinomycin D. | en_US |
dc.format.extent | 809249 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Stimulatory effect of gonadotropins on the synthesis of adenosine 3' :5'-cyclic monophosphate and progesterone by suspensions of rat ovarian interstitial cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Reproductive Endocrinology Program, Departments of Obstetrics and Cynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Reproductive Endocrinology Program, Departments of Obstetrics and Cynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Reproductive Endocrinology Program, Departments of Obstetrics and Cynecology and Biological Chemistry, The University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.identifier.pmid | 164926 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22102/1/0000526.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-4165(75)90077-X | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.